{"id":"f4206d27-790a-5899-b365-82ec707ec152","stable_key":"0f17db03-207f-5910-ac8e-13dfc2f378ce:intestinal-trpm6-loss-low-magnesium","predicate":"reduces","statement":"Intestine-restricted Trpm6 deletion reduced serum and bone magnesium in the tested adult mice.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"20709612-72b4-5d5e-b262-313ae4883378","mechanism_event_label":"Removing intestinal TRPM6 impaired systemic magnesium supply despite intact kidneys.","subject":{"id":"e0283524-8bf3-5a71-8d1b-4dfc4d53df4e","slug":"mouse-intestinal-trpm6-null-genotype","display_name":"Intestine-specific Trpm6-null mouse genotype","entity_type_key":"gene"},"object":{"id":"623a99ab-787c-5d78-8072-72cfcd82008e","slug":"serum-magnesium-concentration","display_name":"Serum magnesium concentration","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"20709612-72b4-5d5e-b262-313ae4883378","stable_key":"0f17db03-207f-5910-ac8e-13dfc2f378ce:intestinal-trpm6-loss-low-magnesium-event","event_type":"biochemical_relationship","label":"Removing intestinal TRPM6 impaired systemic magnesium supply despite intact kidneys.","description":"Intestine-restricted Trpm6 deletion reduced serum and bone magnesium in the tested adult mice.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"877a2fb0-067c-51ca-afea-f63ff16affc8","slug":"trpm6","display_name":"TRPM6","entity_type_key":"protein"},"role":"deleted-channel","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"1423c5f4-b9c1-52ed-9a82-19e5aeece6cb","slug":"intestinal-magnesium-absorption","display_name":"Intestinal magnesium absorption","entity_type_key":"cellular_process"},"role":"impaired-process","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"role":"affected-ion","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e0283524-8bf3-5a71-8d1b-4dfc4d53df4e","slug":"mouse-intestinal-trpm6-null-genotype","display_name":"Intestine-specific Trpm6-null mouse genotype","entity_type_key":"gene"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"623a99ab-787c-5d78-8072-72cfcd82008e","slug":"serum-magnesium-concentration","display_name":"Serum magnesium concentration","entity_type_key":"cellular_process"},"role":"object","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; 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serum and bone","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Intestinal epithelium; serum and bone","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"d0b1be8c-3786-520b-b17d-3c1aa07c853a","evidence_kind":"source_excerpt","locator":"Lines 923-934","start_line":923,"end_line":934,"excerpt":"### intestinal-trpm6-loss-low-magnesium\nIntestine-restricted Trpm6 deletion reduced serum and bone magnesium in the tested adult mice.\nCondition category: machinery_impairment\nnutrient_topic: Magnesium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Removing intestinal TRPM6 impaired systemic magnesium supply despite intact kidneys.\norganism: Mouse\ntissue_or_cell_type: Intestinal epithelium; serum and bone\nexperimental_model: Villin1-Cre conditional deletion; six-month-old male mice\nlimitations: Colon expression was detected; the outcome does not quantify the contribution of each human intestinal segment.\nevidence-system: Villin1-Cre conditional deletion; six-month-old male mice\ntissue: Intestinal epithelium; serum and bone\n[chubanov-2016-trpm6] Epithelial magnesium transport by TRPM6 is essential for prenatal development and adult survival (2016). https://elifesciences.org/articles/20914 DOI: 10.7554/eLife.20914","model_system":"Villin1-Cre conditional deletion; six-month-old male mice","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. 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