{"id":"f4172ff2-e6b4-508e-91de-ae4af1165a10","stable_key":"74ef5bba-ee14-531c-b1bf-d1f297c56bb6:fisetin-rat-respiration","predicate":"reduces","statement":"Fisetin reduced respiratory control and ADP/O ratios and inhibited ADP-stimulated respiration in isolated rat mitochondria.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"cbc47ccf-062b-5bbc-b65e-f9c8b4376eea","mechanism_event_label":"Energy conversion can be impaired at experimental exposures.","subject":{"id":"dee05061-c3f3-5226-8054-dd58b76a585d","slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"},"object":{"id":"5da53c47-a18c-548f-a93e-e999ea89529b","slug":"rat-mitochondrial-coupled-respiration","display_name":"Rat liver mitochondrial coupled respiration","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"cbc47ccf-062b-5bbc-b65e-f9c8b4376eea","stable_key":"74ef5bba-ee14-531c-b1bf-d1f297c56bb6:fisetin-rat-respiration-event","event_type":"observed_relationship","label":"Energy conversion can be impaired at experimental exposures.","description":"Fisetin reduced respiratory control and ADP/O ratios and inhibited ADP-stimulated respiration in isolated rat mitochondria.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"dee05061-c3f3-5226-8054-dd58b76a585d","slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"5da53c47-a18c-548f-a93e-e999ea89529b","slug":"rat-mitochondrial-coupled-respiration","display_name":"Rat liver mitochondrial coupled respiration","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"136c3764-1b93-5f79-8673-9001cab9bc3d","slug":"adp","display_name":"Adenosine diphosphate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Alpha-ketoglutarate- or succinate-supported respiration.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Suggests effects on energy transduction; not direct structural proof of ATP-synthase binding.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Fisetin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Energy conversion can be impaired at experimental exposures.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Prooxidant activity of fisetin: effects on energy metabolism in the rat liver. · 2011 · https://pubmed.ncbi.nlm.nih.gov/20957679/ · DOI 10.1002/jbt.20367","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"701a5633-49cd-5b54-a776-c68667bb65cb","evidence_kind":"source_excerpt","locator":"Lines 336-342","start_line":336,"end_line":342,"excerpt":"## fisetin-rat-respiration\nEnergy conversion can be impaired at experimental exposures.\nFisetin reduced respiratory control and ADP/O ratios and inhibited ADP-stimulated respiration in isolated rat mitochondria.\nModel: Alpha-ketoglutarate- or succinate-supported respiration.\nLimitations: Suggests effects on energy transduction; not direct structural proof of ATP-synthase binding.\nEvidence access: Primary abstract\nProoxidant activity of fisetin: effects on energy metabolism in the rat liver. · 2011 · https://pubmed.ncbi.nlm.nih.gov/20957679/ · DOI 10.1002/jbt.20367","model_system":"Alpha-ketoglutarate- or succinate-supported respiration.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"1108bfff-6504-5152-a51f-ff3d7c27c282","stable_key":"import-74ef5bba-ee14-531c-b1bf-d1f297c56bb6","title":"Fisetin: metabolism, cell-state responses and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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