{"id":"f40e006b-1cf8-53f7-bcf0-bf273216c92b","stable_key":"6cdb37aa-8998-5ea8-829d-4f995caf98fc:l-carnitine-cnta-cleavage","predicate":"with_cntb_cleaves","statement":"Acinetobacter baumannii CntA/CntB catalyzed oxygen-dependent carnitine cleavage to TMA and malic semialdehyde.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"9987cbbe-23e9-5597-b8d3-684a6b7322db","mechanism_event_label":"A separate microbial route needs oxygen.","subject":{"id":"eba25fb7-ae7f-5b0f-8a5d-b6b658c55657","slug":"a-baumannii-cnta","display_name":"Acinetobacter baumannii CntA carnitine monooxygenase component","entity_type_key":"protein"},"object":{"id":"6e34c035-9371-578f-b799-fd2a14e9e40f","slug":"l-carnitine","display_name":"L-Carnitine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"9987cbbe-23e9-5597-b8d3-684a6b7322db","stable_key":"6cdb37aa-8998-5ea8-829d-4f995caf98fc:l-carnitine-cnta-cleavage-event","event_type":"observed_relationship","label":"A separate microbial route needs oxygen.","description":"Acinetobacter baumannii CntA/CntB catalyzed oxygen-dependent carnitine cleavage to TMA and malic semialdehyde.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"eba25fb7-ae7f-5b0f-8a5d-b6b658c55657","slug":"a-baumannii-cnta","display_name":"Acinetobacter baumannii CntA carnitine monooxygenase component","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6e34c035-9371-578f-b799-fd2a14e9e40f","slug":"l-carnitine","display_name":"L-Carnitine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"96e90bd3-7403-5b82-8743-896d14024eb1","slug":"a-baumannii-cntb","display_name":"Acinetobacter baumannii CntB reductase component","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"ffdcd3fa-0e47-5c2b-96e2-00b7d1d5674e","slug":"trimethylamine","display_name":"Trimethylamine / TMA","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified bacterial enzyme complex, spectroscopy and mutagenesis.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This aerobic chemistry is not assumed to dominate the anoxic colon.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Carnitine collection; isomer, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-carnitine","display_name":"L-Carnitine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A separate microbial route needs oxygen.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Carnitine metabolism in the human gut: characterization of the two-component carnitine monooxygenase CntAB from Acinetobacter baumannii. · 2020 · https://pubmed.ncbi.nlm.nih.gov/32694223/ · DOI 10.1074/jbc.RA120.014266","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"bd609f9f-5d7b-57e0-8d41-2aa7b8f4f7d7","evidence_kind":"source_excerpt","locator":"Lines 386-392","start_line":386,"end_line":392,"excerpt":"## l-carnitine-cnta-cleavage\nA separate microbial route needs oxygen.\nAcinetobacter baumannii CntA/CntB catalyzed oxygen-dependent carnitine cleavage to TMA and malic semialdehyde.\nModel: Purified bacterial enzyme complex, spectroscopy and mutagenesis.\nLimitations: This aerobic chemistry is not assumed to dominate the anoxic colon.\nEvidence access: Primary abstract\nCarnitine metabolism in the human gut: characterization of the two-component carnitine monooxygenase CntAB from Acinetobacter baumannii. · 2020 · https://pubmed.ncbi.nlm.nih.gov/32694223/ · DOI 10.1074/jbc.RA120.014266","model_system":"Purified bacterial enzyme complex, spectroscopy and mutagenesis.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e2c9c42b-806a-55e2-abde-b0320ea94704","stable_key":"import-6cdb37aa-8998-5ea8-829d-4f995caf98fc","title":"L-Carnitine: synthesis, acyl-group transport, fuel selection and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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