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(2014). https://pubmed.ncbi.nlm.nih.gov/24790029/ DOI: 10.1126/science.1252826","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Plasma membrane","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"ba2986a2-d914-5b9d-8668-4cfe47a26ca7","evidence_kind":"source_excerpt","locator":"Lines 471-482","start_line":471,"end_line":482,"excerpt":"### chloride-lrrc8a-vrac\nDeleting LRRC8A abolished VRAC currents; reconstitution required LRRC8A with another LRRC8 isoform.\nCondition category: normal\nnutrient_topic: Chloride research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A swelling-responsive anion channel is a protein assembly, not one interchangeable subunit.\norganism: Human cell lines\ntissue_or_cell_type: Plasma membrane\nexperimental_model: Genome-wide screen, gene disruption and rescue\nlimitations: Channel composition and cellular volume response; no dietary intervention.\nexposure: LRRC8 knockout and isoform coexpression\nevidence_span: {\"source_cache\": \"artifacts/chloride-research/24790029.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"9fc0ba50276b74ac5cd7aa29c4c8af4917132a43259b45363b5c16a19b775334\", \"start_char\": 0, \"end_char\": 896, \"text_sha256\": \"9fc0ba50276b74ac5cd7aa29c4c8af4917132a43259b45363b5c16a19b775334\"}\n[chloride-p24790029] Identification of LRRC8 heteromers as an essential component of the volume-regulated anion channel VRAC. 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