{"id":"f38185f7-6bd6-5156-81eb-37f5e1ce1e21","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-met-kynu-hydrolysis","predicate":"catalyzes-hydrolysis-of","statement":"Human KYNU cleaves 3-hydroxykynurenine to 3-hydroxyanthranilate and alanine using PLP.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"29fc710e-047f-5bfa-800e-a8a9bc495678","mechanism_event_label":"This B6-dependent step processes the product of upstream KMO chemistry.","subject":{"id":"573bd034-6b1a-585e-9421-25ca28510d89","slug":"kynu","display_name":"Human kynureninase / KYNU","entity_type_key":"protein"},"object":{"id":"215d55a2-1657-589c-aaf4-afd1957859e7","slug":"3-hydroxykynurenine","display_name":"3-Hydroxy-L-kynurenine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"29fc710e-047f-5bfa-800e-a8a9bc495678","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-met-kynu-hydrolysis-event","event_type":"biochemical_relationship","label":"This B6-dependent step processes the product of upstream KMO chemistry.","description":"Human KYNU cleaves 3-hydroxykynurenine to 3-hydroxyanthranilate and alanine using PLP.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"573bd034-6b1a-585e-9421-25ca28510d89","slug":"kynu","display_name":"Human kynureninase / KYNU","entity_type_key":"protein"},"role":"enzyme","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"role":"cofactor","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"215d55a2-1657-589c-aaf4-afd1957859e7","slug":"3-hydroxykynurenine","display_name":"3-Hydroxy-L-kynurenine","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"f73f064a-cc62-5755-8563-41ef84e86df9","slug":"3-hydroxyanthranilate","display_name":"3-Hydroxyanthranilate","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"ebdc4461-c059-563e-88b0-422c21fdaa25","slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"d31225b7-8e94-5857-9ea1-67ac794b1f15","slug":"water","display_name":"H2O","entity_type_key":"small_molecule"},"role":"reactant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""},{"entity":{"id":"5769b5e3-bbb8-5d36-a877-eeb3e3e27b31","slug":"kmo","display_name":"Human kynurenine 3-monooxygenase / KMO","entity_type_key":"protein"},"role":"upstream enzyme","stoichiometry":null,"state_label":"","sequence_order":6,"notes":""},{"entity":{"id":"e2cd7179-f218-54e8-9ce9-7a836ae35fac","slug":"fad","display_name":"FAD","entity_type_key":"small_molecule"},"role":"upstream KMO cofactor","stoichiometry":null,"state_label":"","sequence_order":7,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"PLP-dependent KYNU follows the FAD-dependent KMO step, connecting B6 and B2 with tryptophan-niacin metabolism.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"existing_related_claim_ids","value_text":"[\"5570a502-6ebf-5397-bf2a-0148727e7695\"]","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant human KYNU crystallography and biochemical characterization","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"KYNU does not directly make NAD; subsequent enzymes and dietary niacin also contribute.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-b6","display_name":"Vitamin B6","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"This B6-dependent step processes the product of upstream KMO chemistry.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b6-kynu-2007] Crystal Structure of Homo Sapiens Kynureninase (2007). https://pmc.ncbi.nlm.nih.gov/articles/PMC2531291/ DOI: 10.1021/bi0616697","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified recombinant protein; no intact tissue","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"9e915424-053c-5e9f-9e97-e9befc29cabb","evidence_kind":"source_excerpt","locator":"Lines 885-896","start_line":885,"end_line":896,"excerpt":"### b6-met-kynu-hydrolysis\nHuman KYNU cleaves 3-hydroxykynurenine to 3-hydroxyanthranilate and alanine using PLP.\nCondition category: normal\nnutrient_topic: Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: This B6-dependent step processes the product of upstream KMO chemistry.\norganism: Homo sapiens\ntissue_or_cell_type: Purified recombinant protein; no intact tissue\nexperimental_model: Recombinant human KYNU crystallography and biochemical characterization\nlimitations: KYNU does not directly make NAD; subsequent enzymes and dietary niacin also contribute.\ncross_nutrient: PLP-dependent KYNU follows the FAD-dependent KMO step, connecting B6 and B2 with tryptophan-niacin metabolism.\nexisting_related_claim_ids: [\"5570a502-6ebf-5397-bf2a-0148727e7695\"]\n[b6-kynu-2007] Crystal Structure of Homo Sapiens Kynureninase (2007). https://pmc.ncbi.nlm.nih.gov/articles/PMC2531291/ DOI: 10.1021/bi0616697","model_system":"Recombinant human KYNU crystallography and biochemical characterization","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [b6-kynu-2007] Crystal Structure of Homo Sapiens Kynureninase (2007). https://pmc.ncbi.nlm.nih.gov/articles/PMC2531291/ DOI: 10.1021/bi0616697","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"251773bb-16f5-5903-b135-db4a61d9dec4","stable_key":"import-1310afbd-6010-586e-805d-551d846da421","title":"Vitamin B6: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"ef0019b344b2219220f801a84d0d138ff6880c1be1a59540d9034bfa4334f61e","revision_id":"cac3555f-48af-5c52-a84e-add4482c87fb","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}