{"id":"f345ad55-8b14-527e-9bc8-cefec321c2eb","stable_key":"b428117d-3a8c-5e74-8711-168153e19545:aspartame-anpep-specificity","predicate":"does_not_hydrolyze_tested","statement":"Purified aminopeptidase N did not hydrolyze aspartame despite partial inhibition of membrane metabolism by actinonin.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"2e8eab8c-a0ec-5273-8c36-df0d95613769","mechanism_event_label":"An inhibitor result alone can misidentify the enzyme.","subject":{"id":"81f32d55-d88b-5009-ae98-e9ed7f62f04c","slug":"1994-purified-aminopeptidase-n","display_name":"Purified aminopeptidase N in the 1994 study, species unresolved","entity_type_key":"protein"},"object":{"id":"15bd1664-9d4b-514f-ab83-87274a67673f","slug":"aspartame","display_name":"Aspartame","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"2e8eab8c-a0ec-5273-8c36-df0d95613769","stable_key":"b428117d-3a8c-5e74-8711-168153e19545:aspartame-anpep-specificity-event","event_type":"observed_relationship","label":"An inhibitor result alone can misidentify the enzyme.","description":"Purified aminopeptidase N did not hydrolyze aspartame despite partial inhibition of membrane metabolism by actinonin.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"81f32d55-d88b-5009-ae98-e9ed7f62f04c","slug":"1994-purified-aminopeptidase-n","display_name":"Purified aminopeptidase N in the 1994 study, species unresolved","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"15bd1664-9d4b-514f-ab83-87274a67673f","slug":"aspartame","display_name":"Aspartame","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified preparation versus membrane assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Species of purified preparation unresolved in accessed abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Aspartame collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"aspartame","display_name":"Aspartame","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"An inhibitor result alone can misidentify the enzyme.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Metabolism of aspartame by human and pig intestinal microvillar peptidases. · 1994 · https://pubmed.ncbi.nlm.nih.gov/8141778/ · DOI 10.1042/bj2980635","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"dd69cb99-3c20-5d4a-b482-31a66d24b565","evidence_kind":"source_excerpt","locator":"Lines 66-72","start_line":66,"end_line":72,"excerpt":"## aspartame-anpep-specificity\nAn inhibitor result alone can misidentify the enzyme.\nPurified aminopeptidase N did not hydrolyze aspartame despite partial inhibition of membrane metabolism by actinonin.\nModel: Purified preparation versus membrane assays.\nLimitations: Species of purified preparation unresolved in accessed abstract.\nEvidence access: Primary abstract\nMetabolism of aspartame by human and pig intestinal microvillar peptidases. · 1994 · https://pubmed.ncbi.nlm.nih.gov/8141778/ · DOI 10.1042/bj2980635","model_system":"Purified preparation versus membrane assays.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2bba7d36-993f-5ba7-acaf-2e4d573527ed","stable_key":"import-b428117d-3a8c-5e74-8711-168153e19545","title":"Aspartame: digestion, taste, metabolite dependencies and experimental signaling (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"21cfc6808062c6ea1fb5d34128bcd005d31f0f6c0d63484794238db77d32ae97","revision_id":"1255dda8-df99-5d4b-a65b-4144c47d7d9b","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}