{"id":"f32af76e-a33d-5df6-b64c-007b2790d848","stable_key":"3c852f36-7951-5fd9-9013-d771a8d16d8a:vae-rgr-photoisomerization","predicate":"photoisomerizes_to","statement":"Illuminating native bovine RGR converted its bound all-trans-retinal stereospecifically to the 11-cis configuration.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"90a6cad7-8335-5f4b-8890-89e8e641125b","mechanism_event_label":"Light lets RGR reverse the retinal isomer change used in visual pigments.","subject":{"id":"194eccf2-c7a5-52a6-a0c6-1c1f6d0f3a3e","slug":"all-trans-retinal","display_name":"All-trans-retinal","entity_type_key":"small_molecule"},"object":{"id":"2df0d238-71af-5f0b-8359-cef18235c4af","slug":"11-cis-retinal","display_name":"11-cis-retinal","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"90a6cad7-8335-5f4b-8890-89e8e641125b","stable_key":"3c852f36-7951-5fd9-9013-d771a8d16d8a:vae-rgr-photoisomerization-event","event_type":"biochemical_relationship","label":"Light lets RGR reverse the retinal isomer change used in visual pigments.","description":"Illuminating native bovine RGR converted its bound all-trans-retinal stereospecifically to the 11-cis configuration.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"205eda81-4688-5fa3-a08c-e94d0c1cbf5c","slug":"rgr","display_name":"Retinal G protein-coupled receptor / RGR opsin","entity_type_key":"protein"},"role":"photoisomerase","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"194eccf2-c7a5-52a6-a0c6-1c1f6d0f3a3e","slug":"all-trans-retinal","display_name":"All-trans-retinal","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"2df0d238-71af-5f0b-8359-cef18235c4af","slug":"11-cis-retinal","display_name":"11-cis-retinal","entity_type_key":"small_molecule"},"role":"photoproduct","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Abstract: irradiation and isomer analysis","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified native RGR photochemistry","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"470-nm monochromatic or near-UV irradiation; duration absent from the abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Abstract-limited; the finding does not establish relative flux in living human eyes.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient","value_text":"Vitamin A","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-a","display_name":"Vitamin A","entity_type_key":"chemical_species"}},{"dimension":"nutrient_topic","value_text":"Vitamin A expansion research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-a","display_name":"Vitamin A","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Bos taurus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Light lets RGR reverse the retinal isomer change used in visual pigments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[vav-hao1999] The endogenous chromophore of retinal G protein-coupled receptor opsin from the pigment epithelium. (1999). https://pubmed.ncbi.nlm.nih.gov/10037690/ DOI: 10.1074/jbc.274.10.6085","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"RPE-derived protein","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"68ec9dd7-0b0b-555f-a642-fd843721e1df","evidence_kind":"source_excerpt","locator":"Lines 423-436","start_line":423,"end_line":436,"excerpt":"### vae-rgr-photoisomerization\nIlluminating native bovine RGR converted its bound all-trans-retinal stereospecifically to the 11-cis configuration.\nCondition category: normal\nnutrient_topic: Vitamin A expansion research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Light lets RGR reverse the retinal isomer change used in visual pigments.\norganism: Bos taurus\ntissue_or_cell_type: RPE-derived protein\nexperimental_model: Purified native RGR photochemistry\nlimitations: Abstract-limited; the finding does not establish relative flux in living human eyes.\nexposure: 470-nm monochromatic or near-UV irradiation; duration absent from the abstract.\ncross_nutrient: false\nevidence_location: Abstract: irradiation and isomer analysis\nnutrient: Vitamin A\n[vav-hao1999] The endogenous chromophore of retinal G protein-coupled receptor opsin from the pigment epithelium. 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