{"id":"f232a845-4bd3-5103-bc63-9bbd740143c9","stable_key":"4df6a932-097e-563a-91fe-5dceec471ffd:chloride-clc7-bone","predicate":"loss_of_function_impairs","statement":"Clcn7-null mice had severe osteopetrosis despite normal osteoclast numbers.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"2529ad0a-fa07-59b6-8d51-71d2d425692b","mechanism_event_label":"Having bone-resorbing cells is insufficient when their transport machinery fails.","subject":{"id":"14ecbb64-fb3a-5d62-bf5d-d0f8de309c92","slug":"mouse-clcn7","display_name":"Mouse ClC-7 chloride/proton exchanger / Clcn7","entity_type_key":"protein"},"object":{"id":"0adc6543-b0c1-5a49-9428-80f727ffeb9a","slug":"bone-resorption","display_name":"Osteoclastic bone resorption","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"2529ad0a-fa07-59b6-8d51-71d2d425692b","stable_key":"4df6a932-097e-563a-91fe-5dceec471ffd:chloride-clc7-bone-event","event_type":"biochemical_relationship","label":"Having bone-resorbing cells is insufficient when their transport machinery fails.","description":"Clcn7-null mice had severe osteopetrosis despite normal osteoclast numbers.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"bone_mineral_component","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"ee63fe8e-92f3-552f-8049-a5f1fa1e1523","slug":"phosphate-ion","display_name":"Inorganic phosphate (Pi; protonation depends on pH)","entity_type_key":"ion"},"role":"bone_mineral_component","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"14ecbb64-fb3a-5d62-bf5d-d0f8de309c92","slug":"mouse-clcn7","display_name":"Mouse ClC-7 chloride/proton exchanger / Clcn7","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"0adc6543-b0c1-5a49-9428-80f727ffeb9a","slug":"bone-resorption","display_name":"Osteoclastic bone resorption","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/chloride-research/11207362.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"5e10ae61257fa402ab9e10d55af44013139462a9d85236b75f5b52bdea0c7b5a\", \"start_char\": 0, \"end_char\": 821, \"text_sha256\": \"5e10ae61257fa402ab9e10d55af44013139462a9d85236b75f5b52bdea0c7b5a\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Clcn7 knockout and human mutation identification","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"ClC-7 loss","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Historical title calls ClC-7 a channel; current exchanger classification does not change the reported knockout phenotype. Not a dietary chloride study.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Chloride research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"chloride","display_name":"Chloride","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Mouse; human infantile osteopetrosis patient","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Having bone-resorbing cells is insufficient when their transport machinery fails.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[chloride-p11207362] Loss of the ClC-7 chloride channel leads to osteopetrosis in mice and man. (2001). https://pubmed.ncbi.nlm.nih.gov/11207362/ DOI: 10.1016/s0092-8674(01)00206-9","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Osteoclast ruffled border","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"f513cc94-503a-5dc4-8a17-2341bcf27110","evidence_kind":"source_excerpt","locator":"Lines 783-794","start_line":783,"end_line":794,"excerpt":"### chloride-clc7-bone\nClcn7-null mice had severe osteopetrosis despite normal osteoclast numbers.\nCondition category: machinery_impairment\nnutrient_topic: Chloride research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Having bone-resorbing cells is insufficient when their transport machinery fails.\norganism: Mouse; human infantile osteopetrosis patient\ntissue_or_cell_type: Osteoclast ruffled border\nexperimental_model: Clcn7 knockout and human mutation identification\nlimitations: Historical title calls ClC-7 a channel; current exchanger classification does not change the reported knockout phenotype. Not a dietary chloride study.\nexposure: ClC-7 loss\nevidence_span: {\"source_cache\": \"artifacts/chloride-research/11207362.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"5e10ae61257fa402ab9e10d55af44013139462a9d85236b75f5b52bdea0c7b5a\", \"start_char\": 0, \"end_char\": 821, \"text_sha256\": \"5e10ae61257fa402ab9e10d55af44013139462a9d85236b75f5b52bdea0c7b5a\"}\n[chloride-p11207362] Loss of the ClC-7 chloride channel leads to osteopetrosis in mice and man. (2001). https://pubmed.ncbi.nlm.nih.gov/11207362/ DOI: 10.1016/s0092-8674(01)00206-9","model_system":"Clcn7 knockout and human mutation identification","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [chloride-p11207362] Loss of the ClC-7 chloride channel leads to osteopetrosis in mice and man. (2001). https://pubmed.ncbi.nlm.nih.gov/11207362/ DOI: 10.1016/s0092-8674(01)00206-9","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"1c2070d5-9f15-5945-b325-d0b49a32c04b","stable_key":"import-4df6a932-097e-563a-91fe-5dceec471ffd","title":"Chloride: transport, acid-base balance, nutrient interactions and loss states (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e32e796f293a3287c6b8f4394881db94cc741ba96e3166e44da2dd14f208f645","revision_id":"8d5fd8ef-db54-5198-a368-23329a02d81b","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}