{"id":"f22e59ff-61ea-5e90-bd3c-3b2ff5348c71","stable_key":"ee4227e5-80d7-55e9-ada7-3c5a16f93ac9:cla-dgk-inhibitor","predicate":"attenuates","statement":"DGK inhibitor R59022 attenuated CLA-induced calcium accumulation and reductions in glucose/fatty-acid uptake.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"5d5c5030-f92f-52ab-a727-df82e9fe63de","mechanism_event_label":"Pharmacological results connected lipid signaling and calcium.","subject":{"id":"9f28ccea-0f8e-5bab-86a6-c8d28391ee08","slug":"r59022","display_name":"R59022, pharmacological DGK inhibitor","entity_type_key":"small_molecule"},"object":{"id":"32e78251-e021-5b6f-9366-8be31c98d657","slug":"human-adipocyte-calcium-signal","display_name":"Human adipocyte intracellular calcium response","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"5d5c5030-f92f-52ab-a727-df82e9fe63de","stable_key":"ee4227e5-80d7-55e9-ada7-3c5a16f93ac9:cla-dgk-inhibitor-event","event_type":"observed_relationship","label":"Pharmacological results connected lipid signaling and calcium.","description":"DGK inhibitor R59022 attenuated CLA-induced calcium accumulation and reductions in glucose/fatty-acid uptake.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"9f28ccea-0f8e-5bab-86a6-c8d28391ee08","slug":"r59022","display_name":"R59022, pharmacological DGK inhibitor","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"32e78251-e021-5b6f-9366-8be31c98d657","slug":"human-adipocyte-calcium-signal","display_name":"Human adipocyte intracellular calcium response","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"9d077e57-af75-5199-b040-b5c2acb02c7f","slug":"dgkh","display_name":"Human diacylglycerol kinase eta / DGKH","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"4405f918-c17d-5365-b873-1678989f8ac1","slug":"cla-trans10-cis12","display_name":"trans-10,cis-12 conjugated linoleic acid","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Primary human adipocytes, R59022 pretreatment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The inhibitor does not establish DGK-eta as the calcium-controlling isoform.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"CLA collection; isomer, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"conjugated-linoleic-acid","display_name":"Conjugated linoleic acid / CLA isomer family","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"Pharmacological results connected lipid signaling and calcium.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Diacylglycerol kinase inhibitor R59022 attenuates conjugated linoleic acid-mediated inflammation in human adipocytes. · 2013 · https://pubmed.ncbi.nlm.nih.gov/23264678/ · DOI 10.1194/jlr.M031211","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"ddf811f6-65a6-543d-88c5-f210d63436d9","evidence_kind":"source_excerpt","locator":"Lines 198-204","start_line":198,"end_line":204,"excerpt":"## cla-dgk-inhibitor\nPharmacological results connected lipid signaling and calcium.\nDGK inhibitor R59022 attenuated CLA-induced calcium accumulation and reductions in glucose/fatty-acid uptake.\nModel: Primary human adipocytes, R59022 pretreatment.\nLimitations: The inhibitor does not establish DGK-eta as the calcium-controlling isoform.\nEvidence access: Primary abstract\nDiacylglycerol kinase inhibitor R59022 attenuates conjugated linoleic acid-mediated inflammation in human adipocytes. · 2013 · https://pubmed.ncbi.nlm.nih.gov/23264678/ · DOI 10.1194/jlr.M031211","model_system":"Primary human adipocytes, R59022 pretreatment.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"45cfa6e3-2e7d-57c1-8c35-65302b7b71db","stable_key":"import-ee4227e5-80d7-55e9-ada7-3c5a16f93ac9","title":"Conjugated linoleic acid: isomers, signaling, nutrient interactions and discovery (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"f9fe73a8a4008d52061c65abe6de7a9806da75f1c478c5adace9387b8ffaad9e","revision_id":"21196d9c-aaab-5fe1-8f32-2987c94289c6","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}