{"id":"f2262778-989a-5847-a743-618b072729af","stable_key":"b7014def-74ec-599e-8347-167e24fec1f0:glutamate-dietary-nitrogen","predicate":"supplies_measured_nitrogen_to","statement":"After six fasted adults ingested 100 mg nitrogen-labeled L-glutamic acid, most recovered circulating amino-acid label appeared in alanine and glutamine, with no significant amino-acid concentration changes during 150 minutes.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"891d848e-cf6b-5bb9-b5d7-b0237c37d1e7","mechanism_event_label":"The incoming amino nitrogen could leave the gut region in different molecules.","subject":{"id":"7da684a4-2641-5bc6-93ae-8c6aa384e487","slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"},"object":{"id":"f77f6ce8-c3d9-525c-909c-728bf27ebfac","slug":"human-alanine-glutamine-nitrogen-label","display_name":"Human arterial alanine/glutamine nitrogen labeling","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"891d848e-cf6b-5bb9-b5d7-b0237c37d1e7","stable_key":"b7014def-74ec-599e-8347-167e24fec1f0:glutamate-dietary-nitrogen-event","event_type":"observed_relationship","label":"The incoming amino nitrogen could leave the gut region in different molecules.","description":"After six fasted adults ingested 100 mg nitrogen-labeled L-glutamic acid, most recovered circulating amino-acid label appeared in alanine and glutamine, with no significant amino-acid concentration changes during 150 minutes.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"7da684a4-2641-5bc6-93ae-8c6aa384e487","slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"f77f6ce8-c3d9-525c-909c-728bf27ebfac","slug":"human-alanine-glutamine-nitrogen-label","display_name":"Human arterial alanine/glutamine nitrogen labeling","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ebdc4461-c059-563e-88b0-422c21fdaa25","slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"8637bb39-2c30-5168-baaf-3e613d831db0","slug":"glutamine","display_name":"L-Glutamine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human oral 15N tracer, arterialized blood sampling for 150 minutes.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Small tracer experiment; it does not establish supplement efficacy or a universal conversion fraction.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Glutamate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The incoming amino nitrogen could leave the gut region in different molecules.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Measurement of the transfer of the nitrogen moiety of intestinal lumen glutamic acid in man after oral ingestion of l-[15N]glutamic acid. · 1988 · https://pubmed.ncbi.nlm.nih.gov/2908193/ · DOI 10.1042/cs0750499","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"8a23c2b8-3e65-531e-8316-492194c691c4","evidence_kind":"source_excerpt","locator":"Lines 34-40","start_line":34,"end_line":40,"excerpt":"## glutamate-dietary-nitrogen\nThe incoming amino nitrogen could leave the gut region in different molecules.\nAfter six fasted adults ingested 100 mg nitrogen-labeled L-glutamic acid, most recovered circulating amino-acid label appeared in alanine and glutamine, with no significant amino-acid concentration changes during 150 minutes.\nModel: Human oral 15N tracer, arterialized blood sampling for 150 minutes.\nLimitations: Small tracer experiment; it does not establish supplement efficacy or a universal conversion fraction.\nEvidence access: Primary abstract\nMeasurement of the transfer of the nitrogen moiety of intestinal lumen glutamic acid in man after oral ingestion of l-[15N]glutamic acid. · 1988 · https://pubmed.ncbi.nlm.nih.gov/2908193/ · DOI 10.1042/cs0750499","model_system":"Human oral 15N tracer, arterialized blood sampling for 150 minutes.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"c6a55239-e808-5ed7-9fa3-755a18ecb29f","stable_key":"import-b7014def-74ec-599e-8347-167e24fec1f0","title":"L-Glutamate / L-glutamic acid: carbon and nitrogen allocation, signaling and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"3da83bd75fddeb96d17af72d7e2ae04344779957650f266521f6c8ebd59fb54f","revision_id":"55196c72-94e4-540a-a869-81d35647b04a","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}