{"id":"f1bbe639-61e3-5d74-baa5-f8e316577e09","stable_key":"3d41d18a-e13a-59a0-a081-55bfcdd43e55:ceylon-oil-pxr-hep-null","predicate":"no_detected_activation","statement":"The oil did not activate the PXR reporter in HepG2 cells over the tested range.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"185486dd-c92d-55ec-9699-def58858afaa","mechanism_event_label":"This liver-cell result differed from the intestinal-cell result.","subject":{"id":"3b21d651-c1e3-5427-b568-91b16e7c3263","slug":"ceylon-oil-2025","display_name":"C. verum oil preparation in the 2025 metabolic clearance study","entity_type_key":"chemical_species"},"object":{"id":"f9deea3c-fcb8-51c8-9194-438c2ad6298b","slug":"nr1i2","display_name":"Human pregnane X receptor / SXR / PXR / NR1I2","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"185486dd-c92d-55ec-9699-def58858afaa","stable_key":"3d41d18a-e13a-59a0-a081-55bfcdd43e55:ceylon-oil-pxr-hep-null-event","event_type":"biochemical_relationship","label":"This liver-cell result differed from the intestinal-cell result.","description":"The oil did not activate the PXR reporter in HepG2 cells over the tested range.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"3b21d651-c1e3-5427-b568-91b16e7c3263","slug":"ceylon-oil-2025","display_name":"C. verum oil preparation in the 2025 metabolic clearance study","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"f9deea3c-fcb8-51c8-9194-438c2ad6298b","slug":"nr1i2","display_name":"Human pregnane X receptor / SXR / PXR / NR1I2","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/ceylon-research/39845339.fulltext.txt\", \"locator\": \"Primary full-text span; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"0bbeb0c733ff258e7efaf517438f4368e29bf3afda37cfbac3be149bde094758\", \"start_char\": 27731, \"end_char\": 28275, \"text_sha256\": \"a6199e4f39be87d9019845ab113a787a4f41c373e18c9ba8ed0aeaef771aa7c1\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human receptor reporters and CYP inhibition assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Oil/parent/metabolite comparisons; PXR 0.62–20 micrograms/mL for 24 hours","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Reporter activation is not demonstrated induction of clinical drug clearance. HepG2 is liver-derived; full results distinguish it from intestinal LS174T.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Ceylon cinnamon research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"ceylon","display_name":"Ceylon cinnamon / Cinnamomum verum bark preparations","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Human-derived reporter cells and human CYP assay systems","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"This liver-cell result differed from the intestinal-cell result.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[ceylon-p39845339] Evaluation of bioaccessibility, metabolic clearance and interaction with xenobiotic receptors (PXR and AhR) of cinnamaldehyde. (2025). https://pubmed.ncbi.nlm.nih.gov/39845339/ DOI: 10.1016/j.fochms.2024.100237","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"HepG2 hepatocellular carcinoma cells","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"49cee86b-ab33-5830-a56f-06c87bd2fc63","evidence_kind":"source_excerpt","locator":"Lines 922-933","start_line":922,"end_line":933,"excerpt":"### ceylon-oil-pxr-hep-null\nThe oil did not activate the PXR reporter in HepG2 cells over the tested range.\nCondition category: normal\nnutrient_topic: Ceylon cinnamon research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: This liver-cell result differed from the intestinal-cell result.\norganism: Human-derived reporter cells and human CYP assay systems\ntissue_or_cell_type: HepG2 hepatocellular carcinoma cells\nexperimental_model: Human receptor reporters and CYP inhibition assays\nlimitations: Reporter activation is not demonstrated induction of clinical drug clearance. HepG2 is liver-derived; full results distinguish it from intestinal LS174T.\nexposure: Oil/parent/metabolite comparisons; PXR 0.62–20 micrograms/mL for 24 hours\nevidence_span: {\"source_cache\": \"artifacts/ceylon-research/39845339.fulltext.txt\", \"locator\": \"Primary full-text span; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"0bbeb0c733ff258e7efaf517438f4368e29bf3afda37cfbac3be149bde094758\", \"start_char\": 27731, \"end_char\": 28275, \"text_sha256\": \"a6199e4f39be87d9019845ab113a787a4f41c373e18c9ba8ed0aeaef771aa7c1\"}\n[ceylon-p39845339] Evaluation of bioaccessibility, metabolic clearance and interaction with xenobiotic receptors (PXR and AhR) of cinnamaldehyde. 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