{"id":"f18123d0-3766-54ff-b38a-fd585f28a4fb","stable_key":"10629adf-a816-5b7b-82db-a76bfbb946f2:bg-depolymerising-halves-the-effect","predicate":"modulates","statement":"LDL cholesterol was significantly less with 3 g high molecular weight, 4 g medium molecular weight and 3 g medium molecular weight oat beta-glucan cereals than with the wheat-fiber cereal by 0.21 or 5.5%, 0.26 or 6.5% and 0.19 or 4.7% mmol/L respectively, however the effect of 4 g low molecular weight oat beta-glucan per day at 0.10 mmol/L was not significant, log of molecular weight times the amount solubilized was a significant determinant of LDL cholesterol, and efficacy was reduced by 50% when molecular weight was reduced to 210,000 g/mol.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"633bdf75-f44c-53d7-be60-997ba4412df1","mechanism_event_label":"Cutting the polymer into shorter pieces halved what it did, even at a higher dose.","subject":{"id":"b74a821a-9a50-5bb3-955e-2177930567bc","slug":"glucan-molecular-weight","display_name":"Molecular weight of a glucan polymer","entity_type_key":"cellular_process"},"object":{"id":"ac5f5914-4824-5f9b-93c7-a6b5a52b53af","slug":"ldl-cholesterol-concentration","display_name":"LDL cholesterol concentration","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"633bdf75-f44c-53d7-be60-997ba4412df1","stable_key":"10629adf-a816-5b7b-82db-a76bfbb946f2:bg-depolymerising-halves-the-effect-event","event_type":"biochemical_relationship","label":"Cutting the polymer into shorter pieces halved what it did, even at a higher dose.","description":"LDL cholesterol was significantly less with 3 g high molecular weight, 4 g medium molecular weight and 3 g medium molecular weight oat beta-glucan cereals than with the wheat-fiber cereal by 0.21 or 5.5%, 0.26 or 6.5% and 0.19 or 4.7% mmol/L respectively, however the effect of 4 g low molecular weight oat beta-glucan per day at 0.10 mmol/L was not significant, log of molecular weight times the amount solubilized was a significant determinant of LDL cholesterol, and efficacy was reduced by 50% when molecular weight was reduced to 210,000 g/mol.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"fa30a72e-ed34-59eb-badd-236561a8db88","slug":"oat-beta-glucan","display_name":"Oat mixed-linkage beta-(1->3)/(1->4)-glucan","entity_type_key":"chemical_species"},"role":"studied_preparation","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"979a2602-df04-58ed-9432-b4d8f797ee37","slug":"glucan-viscosity","display_name":"Viscosity developed by a solubilised glucan","entity_type_key":"cellular_process"},"role":"proposed_mediator","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"fca72f53-5c06-5fcb-8a0b-f48514e63762","slug":"glucan-depolymerisation","display_name":"Depolymerisation of a glucan polymer","entity_type_key":"cellular_process"},"role":"attenuating_process","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"b74a821a-9a50-5bb3-955e-2177930567bc","slug":"glucan-molecular-weight","display_name":"Molecular weight of a glucan polymer","entity_type_key":"cellular_process"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"ac5f5914-4824-5f9b-93c7-a6b5a52b53af","slug":"ldl-cholesterol-concentration","display_name":"LDL cholesterol concentration","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"biomarker_context","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/glucan-research/20660224.abstract.txt\", \"locator\": \"Indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"dbd9787f6688f544bb47e44be561d20ec653daf5627f9022ac1302249924daf0\", \"start_char\": 0, \"end_char\": 2139, \"text_sha256\": \"dbd9787f6688f544bb47e44be561d20ec653daf5627f9022ac1302249924daf0\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Double-blind parallel-design multicentre trial randomising 367 people across four molecular weights and two doses","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Extruded cereal delivering 3 or 4 grams per day of oat beta-glucan at molecular weights of 2,210,000, 850,000, 530,000 or 210,000 grams per mole for four weeks","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The comparator is a wheat-fibre cereal and 345 of 367 completed. 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(2010). https://pubmed.ncbi.nlm.nih.gov/20660224/ DOI: 10.3945/ajcn.2010.29174","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Serum LDL cholesterol","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"biomarker_context","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"99e89165-0392-5e2f-9ac6-41d746c42a98","evidence_kind":"source_excerpt","locator":"Lines 515-526","start_line":515,"end_line":526,"excerpt":"### bg-depolymerising-halves-the-effect\nLDL cholesterol was significantly less with 3 g high molecular weight, 4 g medium molecular weight and 3 g medium molecular weight oat beta-glucan cereals than with the wheat-fiber cereal by 0.21 or 5.5%, 0.26 or 6.5% and 0.19 or 4.7% mmol/L respectively, however the effect of 4 g low molecular weight oat beta-glucan per day at 0.10 mmol/L was not significant, log of molecular weight times the amount solubilized was a significant determinant of LDL cholesterol, and efficacy was reduced by 50% when molecular weight was reduced to 210,000 g/mol.\nCondition category: biomarker_context\nnutrient_topic: Beta-glucan research collection; topical membership is not evidence of a direct clinical effect, and each preparation is recorded as its own entity with no family link joining any pair.\nplain_language: Cutting the polymer into shorter pieces halved what it did, even at a higher dose.\norganism: Human\ntissue_or_cell_type: Serum LDL cholesterol\nexperimental_model: Double-blind parallel-design multicentre trial randomising 367 people across four molecular weights and two doses\nlimitations: The comparator is a wheat-fibre cereal and 345 of 367 completed. A nonsignificant estimate in one arm is not a demonstration of no effect.\nexposure: Extruded cereal delivering 3 or 4 grams per day of oat beta-glucan at molecular weights of 2,210,000, 850,000, 530,000 or 210,000 grams per mole for four weeks\nevidence_span: {\"source_cache\": \"artifacts/glucan-research/20660224.abstract.txt\", \"locator\": \"Indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"dbd9787f6688f544bb47e44be561d20ec653daf5627f9022ac1302249924daf0\", \"start_char\": 0, \"end_char\": 2139, \"text_sha256\": \"dbd9787f6688f544bb47e44be561d20ec653daf5627f9022ac1302249924daf0\"}\n[bg-p20660224] Physicochemical properties of oat β-glucan influence its ability to reduce serum LDL cholesterol in humans: a randomized clinical trial. 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