{"id":"f0e573ad-3b95-50a2-a340-d447bf810506","stable_key":"c3df3634-4c3a-5099-a5d9-e4f6344c1084:setd7-h3k4-methylation","predicate":"methylates","statement":"SETD7 transfers a methyl group from SAM to histone H3 Lys4 in peptide assays.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"171c0702-ad2b-5cbf-95a1-7f97946209d5","mechanism_event_label":"SAM provides the methyl group; the protein-bound lysine receives it.","subject":{"id":"0bc82d25-c6b5-5fb4-88e6-838546adf905","slug":"setd7","display_name":"SETD7","entity_type_key":"protein"},"object":{"id":"38a9af4b-5e18-5710-b151-c714c818b3e0","slug":"h3k4","display_name":"Histone H3 unmethylated at K4","entity_type_key":"protein_state"},"evidence_count":1,"mechanism_event":{"id":"171c0702-ad2b-5cbf-95a1-7f97946209d5","stable_key":"c3df3634-4c3a-5099-a5d9-e4f6344c1084:setd7-h3k4-methylation-event","event_type":"biochemical_relationship","label":"SAM provides the methyl group; the protein-bound lysine receives it.","description":"SETD7 transfers a methyl group from SAM to histone H3 Lys4 in peptide assays.","status":"provisional","compartment":{"slug":"nucleus","display_name":"Nucleus"},"participants":[{"entity":{"id":"0bc82d25-c6b5-5fb4-88e6-838546adf905","slug":"setd7","display_name":"SETD7","entity_type_key":"protein"},"role":"enzyme","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"38a9af4b-5e18-5710-b151-c714c818b3e0","slug":"h3k4","display_name":"Histone H3 unmethylated at K4","entity_type_key":"protein_state"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"825f2da2-01bc-5874-a3c6-64f5ac867db5","slug":"s-adenosylmethionine","display_name":"S-Adenosyl-L-methionine","entity_type_key":"small_molecule"},"role":"donor","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e84829b1-0607-558e-8add-aaa3af4747e7","slug":"s-adenosylhomocysteine","display_name":"S-Adenosyl-L-homocysteine","entity_type_key":"small_molecule"},"role":"coproduct","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"37c0f163-3aee-5f82-857e-e083c2dbc425","slug":"h3k4me1","display_name":"Histone H3 monomethylated at K4","entity_type_key":"protein_state"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Purified human SET7/9 structure and H3-peptide assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Peptide activity does not establish SETD7 as the dominant H3K4 writer in every chromatin context. This reaction modifies lysine already in a protein. It does not show that extra oral lysine increases the reaction or improves a clinical outcome.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Human","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"SAM provides the methyl group; the protein-bound lysine receives it.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[setd7-2002] Crystal structure and functional analysis of the histone methyltransferase SET7/9 (2002). https://pubmed.ncbi.nlm.nih.gov/12372304/ DOI: 10.1016/s0092-8674(02)00964-9","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Not specified as a whole tissue; see experimental model.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"dfdd64e7-ad47-5d1e-a98e-5b9979f80e35","evidence_kind":"source_excerpt","locator":"Lines 547-555","start_line":547,"end_line":555,"excerpt":"### setd7-h3k4-methylation\nSETD7 transfers a methyl group from SAM to histone H3 Lys4 in peptide assays.\nPlain language: SAM provides the methyl group; the protein-bound lysine receives it.\nCondition category: normal\norganism: Human\ntissue_or_cell_type: Not specified as a whole tissue; see experimental model.\nexperimental_model: Purified human SET7/9 structure and H3-peptide assays.\nlimitations: Peptide activity does not establish SETD7 as the dominant H3K4 writer in every chromatin context. This reaction modifies lysine already in a protein. It does not show that extra oral lysine increases the reaction or improves a clinical outcome.\n[setd7-2002] Crystal structure and functional analysis of the histone methyltransferase SET7/9 (2002). https://pubmed.ncbi.nlm.nih.gov/12372304/ DOI: 10.1016/s0092-8674(02)00964-9","model_system":"Purified human SET7/9 structure and H3-peptide assays.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact quote from the accompanying curation document, not from publisher text. Original study references: [setd7-2002] Crystal structure and functional analysis of the histone methyltransferase SET7/9 (2002). https://pubmed.ncbi.nlm.nih.gov/12372304/ DOI: 10.1016/s0092-8674(02)00964-9","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"7633bde7-dcc9-5086-91c6-a45eb96857f3","stable_key":"import-c3df3634-4c3a-5099-a5d9-e4f6344c1084","title":"L-Lysine: mechanism-first literature curation (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"93998d47c21525409ba82f1c82ededf2893dff15fbe61c7deffc175b0e298e97","revision_id":"3897e31f-6624-59e1-a053-8a81b7361632","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}