{"id":"f0dca54b-6a6a-5ee5-b822-65be07a6bc29","stable_key":"548ab9d6-3a9b-5bed-879c-17d03813b636:b2-pnpo-pnp-oxidation","predicate":"oxidizes","statement":"Purified human PNPO converted PNP to PLP with a turnover number of 0.19 per second at 37 C.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"f11b9dee-5e9f-5c44-8ca8-816c53332eac","mechanism_event_label":"PNPO changes phosphorylated pyridoxine into active B6.","subject":{"id":"a01d08b9-a749-5bc3-934b-af0b35024b54","slug":"pnpo","display_name":"Pyridoxine 5-prime-phosphate oxidase / PNPO","entity_type_key":"protein"},"object":{"id":"be0df099-29bf-56ce-b60b-6c6cfb912bb1","slug":"pyridoxine-phosphate","display_name":"Pyridoxine 5-prime-phosphate","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"f11b9dee-5e9f-5c44-8ca8-816c53332eac","stable_key":"548ab9d6-3a9b-5bed-879c-17d03813b636:b2-pnpo-pnp-oxidation-event","event_type":"biochemical_relationship","label":"PNPO changes phosphorylated pyridoxine into active B6.","description":"Purified human PNPO converted PNP to PLP with a turnover number of 0.19 per second at 37 C.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"be0df099-29bf-56ce-b60b-6c6cfb912bb1","slug":"pyridoxine-phosphate","display_name":"Pyridoxine 5-prime-phosphate","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"d3eacf85-1a35-546d-bd30-161474dd9691","slug":"fmn","display_name":"Flavin mononucleotide","entity_type_key":"small_molecule"},"role":"redox cofactor","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"a01d08b9-a749-5bc3-934b-af0b35024b54","slug":"pnpo","display_name":"Pyridoxine 5-prime-phosphate oxidase / PNPO","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"B2-dependent oxidation activates one B6 precursor.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Results: kinetic properties; Table 1; FMN-binding structure","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant human PNPO expressed in E. coli; crystallography and purified-enzyme kinetics at 37 C.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Purified-enzyme assay","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Biochemical evidence does not establish a dietary threshold or supplementation benefit.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Riboflavin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"riboflavin","display_name":"Riboflavin (vitamin B2)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"PNPO changes phosphorylated pyridoxine into active B6.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[musayev2003] Structure and properties of recombinant human pyridoxine 5'-phosphate oxidase. (2003). https://pubmed.ncbi.nlm.nih.gov/12824491/ DOI: 10.1110/ps.0356203","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified recombinant enzyme; no intact tissue","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"077c5c0a-164b-5004-a89d-6adda4018755","evidence_kind":"source_excerpt","locator":"Lines 1150-1162","start_line":1150,"end_line":1162,"excerpt":"### b2-pnpo-pnp-oxidation\nPurified human PNPO converted PNP to PLP with a turnover number of 0.19 per second at 37 C.\nCondition category: normal\nnutrient_topic: Riboflavin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: PNPO changes phosphorylated pyridoxine into active B6.\norganism: Homo sapiens\ntissue_or_cell_type: Purified recombinant enzyme; no intact tissue\nexperimental_model: Recombinant human PNPO expressed in E. coli; crystallography and purified-enzyme kinetics at 37 C.\nlimitations: Biochemical evidence does not establish a dietary threshold or supplementation benefit.\nexposure: Purified-enzyme assay\ncross_nutrient: B2-dependent oxidation activates one B6 precursor.\nevidence_location: Results: kinetic properties; Table 1; FMN-binding structure\n[musayev2003] Structure and properties of recombinant human pyridoxine 5'-phosphate oxidase. (2003). https://pubmed.ncbi.nlm.nih.gov/12824491/ DOI: 10.1110/ps.0356203","model_system":"Recombinant human PNPO expressed in E. coli; crystallography and purified-enzyme kinetics at 37 C.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [musayev2003] Structure and properties of recombinant human pyridoxine 5'-phosphate oxidase. (2003). https://pubmed.ncbi.nlm.nih.gov/12824491/ DOI: 10.1110/ps.0356203","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4f7c9578-82bf-5e2d-b5c4-72a79fb4f6af","stable_key":"import-548ab9d6-3a9b-5bed-879c-17d03813b636","title":"Riboflavin: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"680cb6bc8249877f2410f551807f10d390fdd719014137d7414ba3420e29228d","revision_id":"7a61e299-908d-5372-860b-99ed190f9d7a","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}