{"id":"f0b33f4d-52b2-57b4-b83f-59a36ea78d6d","stable_key":"52871722-4aa6-5453-a902-3e76356db327:resveratrol-calcium-chelation","predicate":"reduces","statement":"Intracellular calcium chelation with BAPTA-AM reduced AMPK and ACC phosphorylation induced by 50 micromolar resveratrol in mouse C2C12 myotubes.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"511751e9-1c7a-52a6-957f-aea5902ef6bb","mechanism_event_label":"Removing available signaling calcium weakened the response.","subject":{"id":"283b5c26-78b5-5398-90ba-b8f70b2d234a","slug":"bapta-am","display_name":"BAPTA-AM intracellular calcium chelator","entity_type_key":"small_molecule"},"object":{"id":"871e74d6-6e9e-5e9c-9c6b-f269f59cb02a","slug":"mouse-ampk-complexes","display_name":"Mouse AMPK complexes","entity_type_key":"protein_complex"},"evidence_count":1,"mechanism_event":{"id":"511751e9-1c7a-52a6-957f-aea5902ef6bb","stable_key":"52871722-4aa6-5453-a902-3e76356db327:resveratrol-calcium-chelation-event","event_type":"observed_relationship","label":"Removing available signaling calcium weakened the response.","description":"Intracellular calcium chelation with BAPTA-AM reduced AMPK and ACC phosphorylation induced by 50 micromolar resveratrol in mouse C2C12 myotubes.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"283b5c26-78b5-5398-90ba-b8f70b2d234a","slug":"bapta-am","display_name":"BAPTA-AM intracellular calcium chelator","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"871e74d6-6e9e-5e9c-9c6b-f269f59cb02a","slug":"mouse-ampk-complexes","display_name":"Mouse AMPK complexes","entity_type_key":"protein_complex"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"53549e32-e6b4-5f09-94e6-62867b015713","slug":"resveratrol","display_name":"Resveratrol","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; 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