{"id":"f0aa695d-0921-5d6f-a47e-8b23b1e6f6bb","stable_key":"3c852f36-7951-5fd9-9013-d771a8d16d8a:va-repro-aldh-loss-ovarian-meiosis","predicate":"genetic_disruption_does_not_prevent","statement":"Conditional deletion of Aldh1a1, Aldh1a2 and Aldh1a3 did not prevent fetal ovarian meiotic progression despite strongly reduced RA.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"e4875af7-615f-54cb-8f4c-4b0f4356f76c","mechanism_event_label":"Removing the main RA-synthesis enzymes did not stop fetal ovarian meiosis.","subject":{"id":"c5071ea4-ea36-5763-9ce0-f909977b31b4","slug":"retinoic-acid-biosynthesis","display_name":"All-trans-retinoic-acid biosynthesis","entity_type_key":"cellular_process"},"object":{"id":"bceb97ad-9929-5d6c-8c95-c54f11954938","slug":"mouse-fetal-ovarian-meiotic-progression","display_name":"Mouse fetal ovarian meiotic progression","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"e4875af7-615f-54cb-8f4c-4b0f4356f76c","stable_key":"3c852f36-7951-5fd9-9013-d771a8d16d8a:va-repro-aldh-loss-ovarian-meiosis-event","event_type":"biochemical_relationship","label":"Removing the main RA-synthesis enzymes did not stop fetal ovarian meiosis.","description":"Conditional deletion of Aldh1a1, Aldh1a2 and Aldh1a3 did not prevent fetal ovarian meiotic progression despite strongly reduced RA.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"63693dbb-fe50-5a5d-ba37-7f78dab3f6ea","slug":"aldh1a1","display_name":"Aldehyde dehydrogenase 1A1 / ALDH1A1","entity_type_key":"protein"},"role":"deleted_synthesis_enzyme","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"22f351bd-0efe-52e4-a02c-d23fe7ef780e","slug":"aldh1a2","display_name":"Aldehyde dehydrogenase 1A2 / ALDH1A2","entity_type_key":"protein"},"role":"deleted_synthesis_enzyme","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c8ec4bcc-743c-58fe-8362-4211964ae2cb","slug":"aldh1a3","display_name":"Aldehyde dehydrogenase 1A3 / ALDH1A3","entity_type_key":"protein"},"role":"deleted_synthesis_enzyme","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"05487b29-8c59-5af8-83cf-cf9c79c5ecb8","slug":"all-trans-retinoic-acid","display_name":"All-trans-retinoic acid","entity_type_key":"small_molecule"},"role":"reduced_signal","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"c5071ea4-ea36-5763-9ce0-f909977b31b4","slug":"retinoic-acid-biosynthesis","display_name":"All-trans-retinoic-acid biosynthesis","entity_type_key":"cellular_process"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"bceb97ad-9929-5d6c-8c95-c54f11954938","slug":"mouse-fetal-ovarian-meiotic-progression","display_name":"Mouse fetal ovarian meiotic progression","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Three conditional deletion strategies","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Somatic or ubiquitous Aldh1a1-3 ablation; ovarian analyses around E13.5-E16.5.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Minor Stra8 delay occurred; analytically zero RA from every source was not demonstrated.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin A expansion research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-a","display_name":"Vitamin A","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Removing the main RA-synthesis enzymes did not stop fetal ovarian meiosis.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[chassot2020] Retinoic acid synthesis by ALDH1A proteins is dispensable for meiosis initiation in the mouse fetal ovary. (2020). https://pubmed.ncbi.nlm.nih.gov/32494737/ DOI: 10.1126/sciadv.aaz1261","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"fetal ovary","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"46767b17-0da3-51c1-a303-3bd17bed0a06","evidence_kind":"source_excerpt","locator":"Lines 291-302","start_line":291,"end_line":302,"excerpt":"### va-repro-aldh-loss-ovarian-meiosis\nConditional deletion of Aldh1a1, Aldh1a2 and Aldh1a3 did not prevent fetal ovarian meiotic progression despite strongly reduced RA.\nCondition category: machinery_impairment\nnutrient_topic: Vitamin A expansion research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Removing the main RA-synthesis enzymes did not stop fetal ovarian meiosis.\norganism: Mus musculus\ntissue_or_cell_type: fetal ovary\nexperimental_model: Three conditional deletion strategies\nlimitations: Minor Stra8 delay occurred; analytically zero RA from every source was not demonstrated.\nexposure: Somatic or ubiquitous Aldh1a1-3 ablation; ovarian analyses around E13.5-E16.5.\ncross_nutrient: false\n[chassot2020] Retinoic acid synthesis by ALDH1A proteins is dispensable for meiosis initiation in the mouse fetal ovary. (2020). https://pubmed.ncbi.nlm.nih.gov/32494737/ DOI: 10.1126/sciadv.aaz1261","model_system":"Three conditional deletion strategies","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [chassot2020] Retinoic acid synthesis by ALDH1A proteins is dispensable for meiosis initiation in the mouse fetal ovary. (2020). https://pubmed.ncbi.nlm.nih.gov/32494737/ DOI: 10.1126/sciadv.aaz1261","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"83131a07-353f-5301-9ce6-50754c194952","stable_key":"import-3c852f36-7951-5fd9-9013-d771a8d16d8a","title":"Vitamin A expansion: reproduction, light-driven vision and shared mechanisms (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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