{"id":"f06dc509-f16b-5fd4-b62c-6a952bc60426","stable_key":"10aa417f-4b03-599f-a453-4aa09edeb27c:alanine-neuron-alanine-rescue","predicate":"supports_survival","statement":"Exogenous alanine was necessary for survival of Gpt2-null neurons in culture, while Gpt2-null astrocytes did not show the same requirement.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"f496c195-64ab-550b-9398-ff279edb876a","mechanism_event_label":"Different brain cell types had different needs after the same enzyme loss.","subject":{"id":"ebdc4461-c059-563e-88b0-422c21fdaa25","slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"},"object":{"id":"522d9b1c-eec0-5a13-b564-64ad2f2cd211","slug":"mouse-gpt2-null-neuron-survival","display_name":"Survival of Gpt2-null mouse neurons","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"f496c195-64ab-550b-9398-ff279edb876a","stable_key":"10aa417f-4b03-599f-a453-4aa09edeb27c:alanine-neuron-alanine-rescue-event","event_type":"observed_relationship","label":"Different brain cell types had different needs after the same enzyme loss.","description":"Exogenous alanine was necessary for survival of Gpt2-null neurons in culture, while Gpt2-null astrocytes did not show the same requirement.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ebdc4461-c059-563e-88b0-422c21fdaa25","slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"522d9b1c-eec0-5a13-b564-64ad2f2cd211","slug":"mouse-gpt2-null-neuron-survival","display_name":"Survival of Gpt2-null mouse neurons","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"f78af6f3-4504-551f-b48b-dd25fcf5a629","slug":"mouse-gpt2","display_name":"Mouse mitochondrial alanine aminotransferase / Gpt2","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse neuron and astrocyte cultures with Gpt2 loss.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Culture survival rescue is not proof of full neurological recovery.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Alanine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Different brain cell types had different needs after the same enzyme loss.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Mitochondrial enzyme GPT2 regulates metabolic mechanisms required for neuron growth and motor function in vivo. · 2022 · https://pubmed.ncbi.nlm.nih.gov/34519342/ · DOI 10.1093/hmg/ddab269","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"c2f1eec4-22bb-5956-b1a6-c42fd8386882","evidence_kind":"source_excerpt","locator":"Lines 312-318","start_line":312,"end_line":318,"excerpt":"## alanine-neuron-alanine-rescue\nDifferent brain cell types had different needs after the same enzyme loss.\nExogenous alanine was necessary for survival of Gpt2-null neurons in culture, while Gpt2-null astrocytes did not show the same requirement.\nModel: Mouse neuron and astrocyte cultures with Gpt2 loss.\nLimitations: Culture survival rescue is not proof of full neurological recovery.\nEvidence access: Primary abstract\nMitochondrial enzyme GPT2 regulates metabolic mechanisms required for neuron growth and motor function in vivo. · 2022 · https://pubmed.ncbi.nlm.nih.gov/34519342/ · DOI 10.1093/hmg/ddab269","model_system":"Mouse neuron and astrocyte cultures with Gpt2 loss.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"01c5554c-a8ba-5c86-9b01-a0fa1a886cdb","stable_key":"import-10aa417f-4b03-599f-a453-4aa09edeb27c","title":"L-Alanine: carbon, nitrogen, protein synthesis and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"8ce7dd76c88b2e25fa23d93f9655a244aadb9d90f55ec8b18ecf0fd4625a61d2","revision_id":"4ed32258-0213-599a-a272-6f8f62280773","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}