{"id":"f068e6fd-a5f6-57ec-94e1-6cb3304edb8d","stable_key":"59b18080-c560-563d-abc5-bac4ee82a27c:ergothioneine-antiproteinase-protection","predicate":"preserves","statement":"Ergothioneine limited peroxynitrite-mediated inactivation of alpha-1-antiproteinase in vitro.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"e5cf7b8c-6d20-5de2-9bda-dd456b267e37","mechanism_event_label":"Oxidant defense can preserve a protein function.","subject":{"id":"939c92c3-50ea-5a4a-9654-8e3b94448132","slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"},"object":{"id":"69a39afd-e67d-5b3e-a512-bfbe6a896feb","slug":"alpha1-antiproteinase-assay-preparation","display_name":"Alpha-1-antiproteinase preparation in peroxynitrite assays","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"e5cf7b8c-6d20-5de2-9bda-dd456b267e37","stable_key":"59b18080-c560-563d-abc5-bac4ee82a27c:ergothioneine-antiproteinase-protection-event","event_type":"observed_relationship","label":"Oxidant defense can preserve a protein function.","description":"Ergothioneine limited peroxynitrite-mediated inactivation of alpha-1-antiproteinase in vitro.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"939c92c3-50ea-5a4a-9654-8e3b94448132","slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"69a39afd-e67d-5b3e-a512-bfbe6a896feb","slug":"alpha1-antiproteinase-assay-preparation","display_name":"Alpha-1-antiproteinase preparation in peroxynitrite assays","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Biochemical protein-activity assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Preparation-specific result; no clinical lung-disease benefit established.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Ergothioneine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Oxidant defense can preserve a protein function.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Antioxidant action of ergothioneine: assessment of its ability to scavenge peroxynitrite. · 1997 · https://pubmed.ncbi.nlm.nih.gov/9070285/ · DOI 10.1006/bbrc.1997.6109","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"3cc69da2-9b4b-5142-ada3-edb9e02359b2","evidence_kind":"source_excerpt","locator":"Lines 160-166","start_line":160,"end_line":166,"excerpt":"## ergothioneine-antiproteinase-protection\nOxidant defense can preserve a protein function.\nErgothioneine limited peroxynitrite-mediated inactivation of alpha-1-antiproteinase in vitro.\nModel: Biochemical protein-activity assay.\nLimitations: Preparation-specific result; no clinical lung-disease benefit established.\nEvidence access: Primary abstract\nAntioxidant action of ergothioneine: assessment of its ability to scavenge peroxynitrite. · 1997 · https://pubmed.ncbi.nlm.nih.gov/9070285/ · DOI 10.1006/bbrc.1997.6109","model_system":"Biochemical protein-activity assay.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2f037d98-f3d0-5dbe-80d8-90b738f130d6","stable_key":"import-59b18080-c560-563d-abc5-bac4ee82a27c","title":"Ergothioneine: transport, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"b6def8119f03cfabeec2fb55ba924340dc612e1dd42f05404201fa2b7ced5259","revision_id":"f88c38e6-ffb5-5a68-83ca-4610ca2c2df4","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}