{"id":"f05bf060-2cb6-557d-bdbe-b3dfdd2af160","stable_key":"22527305-af1f-536a-b419-7e73f08cc3ec:luteolin-ck2-plant-structure","predicate":"binds_atp_pocket","statement":"Crystallography located luteolin in the catalytic pocket of Zea mays CK2; kinase inhibition was ATP-competitive.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"12b6b563-3e8c-5cb9-8c7b-9c257ee7b2b9","mechanism_event_label":"The structural experiment used a plant enzyme.","subject":{"id":"80472c4b-ecc2-52e3-a766-f8463294774f","slug":"luteolin","display_name":"Luteolin / 3′,4′,5,7-tetrahydroxyflavone","entity_type_key":"small_molecule"},"object":{"id":"90862c8e-edce-5338-88da-ed73d1899a62","slug":"zea-mays-ck2-catalytic","display_name":"Zea mays CK2 catalytic subunit","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"12b6b563-3e8c-5cb9-8c7b-9c257ee7b2b9","stable_key":"22527305-af1f-536a-b419-7e73f08cc3ec:luteolin-ck2-plant-structure-event","event_type":"observed_relationship","label":"The structural experiment used a plant enzyme.","description":"Crystallography located luteolin in the catalytic pocket of Zea mays CK2; kinase inhibition was ATP-competitive.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"80472c4b-ecc2-52e3-a766-f8463294774f","slug":"luteolin","display_name":"Luteolin / 3′,4′,5,7-tetrahydroxyflavone","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"90862c8e-edce-5338-88da-ed73d1899a62","slug":"zea-mays-ck2-catalytic","display_name":"Zea mays CK2 catalytic subunit","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"CK2 inhibitor kinetics and maize catalytic-subunit crystal structure.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Do not relabel the crystal as human CK2.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Luteolin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"luteolin","display_name":"Luteolin / 3′,4′,5,7-tetrahydroxyflavone","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The structural experiment used a plant enzyme.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Inhibition of protein kinase CK2 by flavonoids and tyrphostins. A structural insight. · 2012 · https://pubmed.ncbi.nlm.nih.gov/22794353/ · DOI 10.1021/bi300531c","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"52b1db69-0937-569a-83b1-9d7f3edd1022","evidence_kind":"source_excerpt","locator":"Lines 340-346","start_line":340,"end_line":346,"excerpt":"## luteolin-ck2-plant-structure\nThe structural experiment used a plant enzyme.\nCrystallography located luteolin in the catalytic pocket of Zea mays CK2; kinase inhibition was ATP-competitive.\nModel: CK2 inhibitor kinetics and maize catalytic-subunit crystal structure.\nLimitations: Do not relabel the crystal as human CK2.\nEvidence access: Primary abstract\nInhibition of protein kinase CK2 by flavonoids and tyrphostins. A structural insight. · 2012 · https://pubmed.ncbi.nlm.nih.gov/22794353/ · DOI 10.1021/bi300531c","model_system":"CK2 inhibitor kinetics and maize catalytic-subunit crystal structure.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"26337a08-5cee-5cee-900e-e6d3e26ac6e4","stable_key":"import-22527305-af1f-536a-b419-7e73f08cc3ec","title":"Luteolin: metabolism, immune signaling, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"fd6ea0b1cef8d1c9965270494bfa815de14f679044f0b5d5d7bbf4904630ad76","revision_id":"c9a3dd80-c7e6-5fb4-bc91-8db265e7c8de","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}