{"id":"f056b029-d712-58dc-b41b-a57b3df6964a","stable_key":"335be270-ea4a-5c8e-ad04-964fd22a439e:vanadium-dtt-speciation","predicate":"converts_pervanadate_to","statement":"Dithiothreitol rapidly converted pervanadate to vanadate under the phosphatase assay conditions.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"f0f7701a-972b-59f2-b26b-ca568a2c2a23","mechanism_event_label":"The reducing environment can change which inhibitor is actually present.","subject":{"id":"d3f1dc28-551f-518b-863f-627b4ac2c5f3","slug":"dithiothreitol","display_name":"Dithiothreitol / DTT","entity_type_key":"small_molecule"},"object":{"id":"bef86a6e-28a0-5df9-9306-3f05991aa159","slug":"vanadate-v","display_name":"Vanadate(V), protonation/speciation dependent","entity_type_key":"chemical_species"},"evidence_count":1,"mechanism_event":{"id":"f0f7701a-972b-59f2-b26b-ca568a2c2a23","stable_key":"335be270-ea4a-5c8e-ad04-964fd22a439e:vanadium-dtt-speciation-event","event_type":"observed_relationship","label":"The reducing environment can change which inhibitor is actually present.","description":"Dithiothreitol rapidly converted pervanadate to vanadate under the phosphatase assay conditions.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"d3f1dc28-551f-518b-863f-627b4ac2c5f3","slug":"dithiothreitol","display_name":"Dithiothreitol / DTT","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"bef86a6e-28a0-5df9-9306-3f05991aa159","slug":"vanadate-v","display_name":"Vanadate(V), protonation/speciation dependent","entity_type_key":"chemical_species"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c5cbad45-22c6-594b-ab6b-71880dab1b24","slug":"vanadium","display_name":"Vanadium","entity_type_key":"nutrient_element"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"f1faad21-091c-5b03-a03c-a20011d36fef","slug":"pervanadate","display_name":"Pervanadate / peroxovanadium(V) assay species","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Cell-free reducing-agent experiment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"DTT is a laboratory reagent; glutathione cannot automatically be assigned identical kinetics.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vanadium collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vanadium","display_name":"Vanadium","entity_type_key":"nutrient_element"}},{"dimension":"plain_language","value_text":"The reducing environment can change which inhibitor is actually present.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Mechanism of inhibition of protein-tyrosine phosphatases by vanadate and pervanadate. · 1997 · https://pubmed.ncbi.nlm.nih.gov/8995372/ · DOI 10.1074/jbc.272.2.843","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"1f25b319-5bda-5709-9193-855d0215759c","evidence_kind":"source_excerpt","locator":"Lines 38-44","start_line":38,"end_line":44,"excerpt":"## vanadium-dtt-speciation\nThe reducing environment can change which inhibitor is actually present.\nDithiothreitol rapidly converted pervanadate to vanadate under the phosphatase assay conditions.\nModel: Cell-free reducing-agent experiment.\nLimitations: DTT is a laboratory reagent; glutathione cannot automatically be assigned identical kinetics.\nEvidence access: Primary abstract\nMechanism of inhibition of protein-tyrosine phosphatases by vanadate and pervanadate. · 1997 · https://pubmed.ncbi.nlm.nih.gov/8995372/ · DOI 10.1074/jbc.272.2.843","model_system":"Cell-free reducing-agent experiment.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e4d5d0ba-529b-540f-96ed-e719d116ccfd","stable_key":"import-335be270-ea4a-5c8e-ad04-964fd22a439e","title":"Vanadium: speciation, phosphate-sensitive enzymes and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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