{"id":"f043f59b-bb4b-5668-be15-7ebaaac50036","stable_key":"434e1e27-eb90-583a-956b-62472726ffba:pth-activates-trpv5","predicate":"activates","statement":"PTH increases TRPV5 activity through cAMP-PKA-dependent phosphorylation at T709 in receptor/channel expression assays.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"4c12e616-e1d9-5629-9a53-dbc4bf06e81e","mechanism_event_label":"PTH can increase calcium entry through TRPV5.","subject":{"id":"e162b0ab-1449-5471-b3c3-4638a0fa3a96","slug":"pth","display_name":"Parathyroid hormone / PTH","entity_type_key":"protein"},"object":{"id":"ae79ed63-956e-5f19-b2bc-0142bb6e9aea","slug":"trpv5","display_name":"TRPV5","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"4c12e616-e1d9-5629-9a53-dbc4bf06e81e","stable_key":"434e1e27-eb90-583a-956b-62472726ffba:pth-activates-trpv5-event","event_type":"biochemical_relationship","label":"PTH can increase calcium entry through TRPV5.","description":"PTH increases TRPV5 activity through cAMP-PKA-dependent phosphorylation at T709 in receptor/channel expression assays.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e162b0ab-1449-5471-b3c3-4638a0fa3a96","slug":"pth","display_name":"Parathyroid hormone / PTH","entity_type_key":"protein"},"role":"hormone","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6ffdecce-6117-5eee-a1b2-f03a6fb6c272","slug":"pth1r","display_name":"Parathyroid hormone receptor 1 / PTH1R","entity_type_key":"protein"},"role":"receptor","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"6cd7f461-9b79-5f75-877f-99910bb84f6c","slug":"pka","display_name":"Protein kinase A","entity_type_key":"protein_family"},"role":"kinase","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"ae79ed63-956e-5f19-b2bc-0142bb6e9aea","slug":"trpv5","display_name":"TRPV5","entity_type_key":"protein"},"role":"channel","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"transported_ion","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"HEK293 expression, calcium uptake, FRET, patch clamp and TRPV5 mutagenesis","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Response required strong intracellular calcium buffering; cultured-cell mechanism.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Calcium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"calcium","display_name":"Calcium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Heterologous HEK293 expression system","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"PTH can increase calcium entry through TRPV5.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[degroot2009] Parathyroid hormone activates TRPV5 via PKA-dependent phosphorylation (2009). https://pmc.ncbi.nlm.nih.gov/articles/PMC2723979/ DOI: 10.1681/ASN.2008080873","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Plasma membrane; distal-nephron mechanism","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"b9b08bf3-99c7-5b06-9bef-e1c4681f20f5","evidence_kind":"source_excerpt","locator":"Lines 58-67","start_line":58,"end_line":67,"excerpt":"### pth-activates-trpv5\nPTH increases TRPV5 activity through cAMP-PKA-dependent phosphorylation at T709 in receptor/channel expression assays.\nCondition category: normal\nnutrient_topic: Calcium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: PTH can increase calcium entry through TRPV5.\norganism: Heterologous HEK293 expression system\ntissue_or_cell_type: Plasma membrane; distal-nephron mechanism\nexperimental_model: HEK293 expression, calcium uptake, FRET, patch clamp and TRPV5 mutagenesis\nlimitations: Response required strong intracellular calcium buffering; cultured-cell mechanism.\n[degroot2009] Parathyroid hormone activates TRPV5 via PKA-dependent phosphorylation (2009). https://pmc.ncbi.nlm.nih.gov/articles/PMC2723979/ DOI: 10.1681/ASN.2008080873","model_system":"HEK293 expression, calcium uptake, FRET, patch clamp and TRPV5 mutagenesis","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [degroot2009] Parathyroid hormone activates TRPV5 via PKA-dependent phosphorylation (2009). https://pmc.ncbi.nlm.nih.gov/articles/PMC2723979/ DOI: 10.1681/ASN.2008080873","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"3c083fa0-59c6-50e9-af42-678d0e3c1006","stable_key":"import-434e1e27-eb90-583a-956b-62472726ffba","title":"Calcium: mechanism-first literature curation (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"965c934d6b147f2b62f8d45ce6d7a87681a600bfe5c46a585b4704889afd3fc1","revision_id":"543ec48b-4f70-5197-9d76-f5a3c443664c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}