{"id":"efec04da-a9c7-54ac-915f-22376d2cceab","stable_key":"22527305-af1f-536a-b419-7e73f08cc3ec:luteolin-pparg-ligand","predicate":"binds","statement":"Luteolin occupied the PPAR gamma ligand pocket in an inactive conformer; myristic acid simultaneously occupied the pocket.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"44eef547-eccb-528e-9043-a92b7fb5a415","mechanism_event_label":"Two ligands fit in the structural preparation.","subject":{"id":"80472c4b-ecc2-52e3-a766-f8463294774f","slug":"luteolin","display_name":"Luteolin / 3′,4′,5,7-tetrahydroxyflavone","entity_type_key":"small_molecule"},"object":{"id":"7f456e18-fabc-532d-bbfe-cd3a8cf1b348","slug":"pparg","display_name":"Human PPAR gamma / PPARG","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"44eef547-eccb-528e-9043-a92b7fb5a415","stable_key":"22527305-af1f-536a-b419-7e73f08cc3ec:luteolin-pparg-ligand-event","event_type":"observed_relationship","label":"Two ligands fit in the structural preparation.","description":"Luteolin occupied the PPAR gamma ligand pocket in an inactive conformer; myristic acid simultaneously occupied the pocket.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"80472c4b-ecc2-52e3-a766-f8463294774f","slug":"luteolin","display_name":"Luteolin / 3′,4′,5,7-tetrahydroxyflavone","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"7f456e18-fabc-532d-bbfe-cd3a8cf1b348","slug":"pparg","display_name":"Human PPAR gamma / PPARG","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"9dff470c-59c4-5a9a-8276-62ffe4c5c925","slug":"myristic-acid","display_name":"Myristic acid","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"PPAR gamma ligand-binding-domain crystallography.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Simulated cooperative stabilization is not demonstrated nutritional synergy.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Luteolin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"luteolin","display_name":"Luteolin / 3′,4′,5,7-tetrahydroxyflavone","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Two ligands fit in the structural preparation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Mode of peroxisome proliferator-activated receptor γ activation by luteolin. · 2012 · https://pubmed.ncbi.nlm.nih.gov/22391103/ · DOI 10.1124/mol.111.076216","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"5ed3587b-14ec-5ca5-9454-be1c52b1b98c","evidence_kind":"source_excerpt","locator":"Lines 364-370","start_line":364,"end_line":370,"excerpt":"## luteolin-pparg-ligand\nTwo ligands fit in the structural preparation.\nLuteolin occupied the PPAR gamma ligand pocket in an inactive conformer; myristic acid simultaneously occupied the pocket.\nModel: PPAR gamma ligand-binding-domain crystallography.\nLimitations: Simulated cooperative stabilization is not demonstrated nutritional synergy.\nEvidence access: Primary abstract\nMode of peroxisome proliferator-activated receptor γ activation by luteolin. · 2012 · https://pubmed.ncbi.nlm.nih.gov/22391103/ · DOI 10.1124/mol.111.076216","model_system":"PPAR gamma ligand-binding-domain crystallography.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"26337a08-5cee-5cee-900e-e6d3e26ac6e4","stable_key":"import-22527305-af1f-536a-b419-7e73f08cc3ec","title":"Luteolin: metabolism, immune signaling, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"fd6ea0b1cef8d1c9965270494bfa815de14f679044f0b5d5d7bbf4904630ad76","revision_id":"c9a3dd80-c7e6-5fb4-bc91-8db265e7c8de","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}