{"id":"efe95897-5f16-584a-aee5-0ae1ef27f69d","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-transport-enterocyte-pn-to-pl","predicate":"is-converted-to","statement":"Polarized Caco-2 cells supplied apically with PN generated PL and released more PL basolaterally than apically.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"244b9257-d2cb-5f80-a7c3-a04ccde07949","mechanism_event_label":"Enterocytes can process a precursor before releasing pyridoxal.","subject":{"id":"aa6648f2-8962-5567-9939-ef4145e40ed1","slug":"pyridoxine","display_name":"Pyridoxine","entity_type_key":"small_molecule"},"object":{"id":"9bf6269d-9e01-553d-bd8b-11a0d613bfca","slug":"pyridoxal","display_name":"Pyridoxal","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"244b9257-d2cb-5f80-a7c3-a04ccde07949","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-transport-enterocyte-pn-to-pl-event","event_type":"biochemical_relationship","label":"Enterocytes can process a precursor before releasing pyridoxal.","description":"Polarized Caco-2 cells supplied apically with PN generated PL and released more PL basolaterally than apically.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"aa6648f2-8962-5567-9939-ef4145e40ed1","slug":"pyridoxine","display_name":"Pyridoxine","entity_type_key":"small_molecule"},"role":"apical precursor","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"9bf6269d-9e01-553d-bd8b-11a0d613bfca","slug":"pyridoxal","display_name":"Pyridoxal","entity_type_key":"small_molecule"},"role":"released product","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"d2d46db7-13a4-595f-adaa-70ad6ca31a6c","slug":"pdxk","display_name":"Pyridoxal kinase / PDXK","entity_type_key":"protein"},"role":"expressed pathway enzyme","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"a01d08b9-a749-5bc3-934b-af0b35024b54","slug":"pnpo","display_name":"Pyridoxine 5-prime-phosphate oxidase / PNPO","entity_type_key":"protein"},"role":"expressed pathway enzyme","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"a0d74853-72b7-546d-a7c7-d37602ba990a","slug":"pdxp","display_name":"Human pyridoxal phosphatase / PDXP","entity_type_key":"protein"},"role":"expressed pathway enzyme","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_location","value_text":"Results: Incubation with pyridoxine; Figure 4","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Polarized human Caco-2 monolayers; human intestinal lysates.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"100 or 1000 nM PN; 6-48 h.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Vitamer measurements establish net conversion; individual enzyme fluxes were not separately perturbed.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-b6","display_name":"Vitamin B6","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Enterocytes can process a precursor before releasing pyridoxal.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[albersen2013] The intestine plays a substantial role in human vitamin B6 metabolism: a Caco-2 cell model. (2013). https://pubmed.ncbi.nlm.nih.gov/23342087/ DOI: 10.1371/journal.pone.0054113","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Polarized Caco-2 monolayers","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"00efa5f5-ffd2-5547-a974-b2c1fe056eae","evidence_kind":"source_excerpt","locator":"Lines 124-135","start_line":124,"end_line":135,"excerpt":"### b6-transport-enterocyte-pn-to-pl\nPolarized Caco-2 cells supplied apically with PN generated PL and released more PL basolaterally than apically.\nCondition category: normal\nnutrient_topic: Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Enterocytes can process a precursor before releasing pyridoxal.\norganism: Homo sapiens\ntissue_or_cell_type: Polarized Caco-2 monolayers\nexperimental_model: Polarized human Caco-2 monolayers; human intestinal lysates.\nlimitations: Vitamer measurements establish net conversion; individual enzyme fluxes were not separately perturbed.\nexposure: 100 or 1000 nM PN; 6-48 h.\nevidence_location: Results: Incubation with pyridoxine; Figure 4\n[albersen2013] The intestine plays a substantial role in human vitamin B6 metabolism: a Caco-2 cell model. (2013). https://pubmed.ncbi.nlm.nih.gov/23342087/ DOI: 10.1371/journal.pone.0054113","model_system":"Polarized human Caco-2 monolayers; human intestinal lysates.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [albersen2013] The intestine plays a substantial role in human vitamin B6 metabolism: a Caco-2 cell model. (2013). https://pubmed.ncbi.nlm.nih.gov/23342087/ DOI: 10.1371/journal.pone.0054113","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"251773bb-16f5-5903-b135-db4a61d9dec4","stable_key":"import-1310afbd-6010-586e-805d-551d846da421","title":"Vitamin B6: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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