{"id":"efc0b59a-b98e-5fb0-9cfc-b038427d5ae5","stable_key":"104835a3-b891-5dc4-a6bb-42ab7c6f96c4:butyrate-osteoclast-metabolism","predicate":"reduces","statement":"Butyrate and propionate shifted osteoclast metabolism toward glycolysis, reduced Traf6/Nfatc1 expression and suppressed osteoclast differentiation in the reported experiments.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"06f7a47f-497e-5acb-91bb-18fd407d60f9","mechanism_event_label":"A separate pathway reduced bone breakdown rather than increasing bone building.","subject":{"id":"e7428a7f-d35a-5406-9904-a88984814833","slug":"butyrate","display_name":"Butyrate","entity_type_key":"small_molecule"},"object":{"id":"d552a7e6-11a9-566e-adb9-4d2491dceb93","slug":"mouse-butyrate-osteoclast-differentiation","display_name":"Mouse osteoclast differentiation after butyrate","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"06f7a47f-497e-5acb-91bb-18fd407d60f9","stable_key":"104835a3-b891-5dc4-a6bb-42ab7c6f96c4:butyrate-osteoclast-metabolism-event","event_type":"observed_relationship","label":"A separate pathway reduced bone breakdown rather than increasing bone building.","description":"Butyrate and propionate shifted osteoclast metabolism toward glycolysis, reduced Traf6/Nfatc1 expression and suppressed osteoclast differentiation in the reported experiments.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e7428a7f-d35a-5406-9904-a88984814833","slug":"butyrate","display_name":"Butyrate","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"d552a7e6-11a9-566e-adb9-4d2491dceb93","slug":"mouse-butyrate-osteoclast-differentiation","display_name":"Mouse osteoclast differentiation after butyrate","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"becbe10f-2b35-55d1-8143-89f76c8df93e","slug":"mouse-traf6","display_name":"Mouse TNF receptor-associated factor 6 / Traf6","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"b4a1bee0-582b-5c47-817f-04b6764cfbe8","slug":"mouse-nfatc1","display_name":"Mouse NFATc1","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse osteoclast cultures and bone-loss interventions.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Distinct from the Treg/Wnt10b bone-formation study; neither proves a universal change in all bone cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Butyrate collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"butyrate","display_name":"Butyrate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A separate pathway reduced bone breakdown rather than increasing bone building.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Short-chain fatty acids regulate systemic bone mass and protect from pathological bone loss. · 2018 · https://pubmed.ncbi.nlm.nih.gov/29302038/ · DOI 10.1038/s41467-017-02490-4","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"c93973b0-383b-522d-a8e7-62ba55f459f9","evidence_kind":"source_excerpt","locator":"Lines 478-484","start_line":478,"end_line":484,"excerpt":"## butyrate-osteoclast-metabolism\nA separate pathway reduced bone breakdown rather than increasing bone building.\nButyrate and propionate shifted osteoclast metabolism toward glycolysis, reduced Traf6/Nfatc1 expression and suppressed osteoclast differentiation in the reported experiments.\nModel: Mouse osteoclast cultures and bone-loss interventions.\nLimitations: Distinct from the Treg/Wnt10b bone-formation study; neither proves a universal change in all bone cells.\nEvidence access: Primary abstract\nShort-chain fatty acids regulate systemic bone mass and protect from pathological bone loss. · 2018 · https://pubmed.ncbi.nlm.nih.gov/29302038/ · DOI 10.1038/s41467-017-02490-4","model_system":"Mouse osteoclast cultures and bone-loss interventions.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"33205bb8-dcbe-5d2c-afca-86075d7748d1","stable_key":"import-104835a3-b891-5dc4-a6bb-42ab7c6f96c4","title":"Butyrate: microbial production, fuel use, signaling and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"a1253eb4426fc45d7b7c113028aa4ba2e9f89a9653272f3795437fc222bad558","revision_id":"c2320e65-a252-57a9-8838-7abf3d23400e","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}