{"id":"ef9e50b2-0523-5111-99ea-2c6067de62fe","stable_key":"7edf94bb-95c8-5234-9161-9eb338bb9b36:boron-cadpr-binding","predicate":"forms_complex_with","statement":"Boric acid bound cyclic ADP-ribose with an apparent association constant of 655 ± 99 L/mol in the electrospray assay at pH 10.3.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"072571e0-f00f-5580-a408-5248ded1e750","mechanism_event_label":"Boron can complex the calcium messenger cADPR under the tested chemical conditions.","subject":{"id":"55ff303e-ae6b-5adf-b34f-a11a53812e9a","slug":"cyclic-adp-ribose","display_name":"Cyclic ADP-ribose","entity_type_key":"small_molecule"},"object":{"id":"fcc8c932-42e6-5618-9bb1-a2147f332e8d","slug":"borate-cadpr-complex","display_name":"Boron complex with cyclic ADP-ribose","entity_type_key":"chemical_species"},"evidence_count":1,"mechanism_event":{"id":"072571e0-f00f-5580-a408-5248ded1e750","stable_key":"7edf94bb-95c8-5234-9161-9eb338bb9b36:boron-cadpr-binding-event","event_type":"biochemical_relationship","label":"Boron can complex the calcium messenger cADPR under the tested chemical conditions.","description":"Boric acid bound cyclic ADP-ribose with an apparent association constant of 655 ± 99 L/mol in the electrospray assay at pH 10.3.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"56db2f3c-f247-573f-bb7c-47cac02ea60f","slug":"boric-acid","display_name":"Boric acid / orthoboric acid / B(OH)3","entity_type_key":"small_molecule"},"role":"binding_partner","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"55ff303e-ae6b-5adf-b34f-a11a53812e9a","slug":"cyclic-adp-ribose","display_name":"Cyclic ADP-ribose","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"fcc8c932-42e6-5618-9bb1-a2147f332e8d","slug":"borate-cadpr-complex","display_name":"Boron complex with cyclic ADP-ribose","entity_type_key":"chemical_species"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/boron-research/16545389.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"720b1c99bd2628157e48db8085947552afc30029faeb65fb9235541b95c4b655\", \"start_char\": 0, \"end_char\": 724, \"text_sha256\": \"720b1c99bd2628157e48db8085947552afc30029faeb65fb9235541b95c4b655\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified Aplysia cyclase kinetics and electrospray complex analysis","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Noncompetitive inhibition Ki 40.5 ± 0.5 mM boric acid; cADPR-binding assay pH 10.3","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The enzyme was Aplysia cyclase, not human CD38. Millimolar enzyme inhibition and alkaline binding data do not establish effects of ordinary dietary boron.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Boron research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"boron","display_name":"Boron","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Aplysia enzyme; cell-free chemistry","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Boron can complex the calcium messenger cADPR under the tested chemical conditions.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[boron-p16545389] Boric acid inhibits adenosine diphosphate-ribosyl cyclase non-competitively. (2006). https://pubmed.ncbi.nlm.nih.gov/16545389/ DOI: 10.1016/j.chroma.2006.02.066","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified enzyme and metabolites","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"616e476c-f34f-55b8-95e3-9bd834cc4601","evidence_kind":"source_excerpt","locator":"Lines 170-181","start_line":170,"end_line":181,"excerpt":"### boron-cadpr-binding\nBoric acid bound cyclic ADP-ribose with an apparent association constant of 655 ± 99 L/mol in the electrospray assay at pH 10.3.\nCondition category: normal\nnutrient_topic: Boron research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Boron can complex the calcium messenger cADPR under the tested chemical conditions.\norganism: Aplysia enzyme; cell-free chemistry\ntissue_or_cell_type: Purified enzyme and metabolites\nexperimental_model: Purified Aplysia cyclase kinetics and electrospray complex analysis\nlimitations: The enzyme was Aplysia cyclase, not human CD38. Millimolar enzyme inhibition and alkaline binding data do not establish effects of ordinary dietary boron.\nexposure: Noncompetitive inhibition Ki 40.5 ± 0.5 mM boric acid; cADPR-binding assay pH 10.3\nevidence_span: {\"source_cache\": \"artifacts/boron-research/16545389.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"720b1c99bd2628157e48db8085947552afc30029faeb65fb9235541b95c4b655\", \"start_char\": 0, \"end_char\": 724, \"text_sha256\": \"720b1c99bd2628157e48db8085947552afc30029faeb65fb9235541b95c4b655\"}\n[boron-p16545389] Boric acid inhibits adenosine diphosphate-ribosyl cyclase non-competitively. (2006). https://pubmed.ncbi.nlm.nih.gov/16545389/ DOI: 10.1016/j.chroma.2006.02.066","model_system":"Purified Aplysia cyclase kinetics and electrospray complex analysis","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [boron-p16545389] Boric acid inhibits adenosine diphosphate-ribosyl cyclase non-competitively. (2006). https://pubmed.ncbi.nlm.nih.gov/16545389/ DOI: 10.1016/j.chroma.2006.02.066","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2d96c547-aeec-5d8b-9d31-1288da209f89","stable_key":"import-7edf94bb-95c8-5234-9161-9eb338bb9b36","title":"Boron: chemistry, nutrient interactions, low-intake studies and mechanistic uncertainties (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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