{"id":"eeec87cd-43b8-5625-a733-99fe88030d7e","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:folate-methyl-gnmt-methyl-use","predicate":"produces","statement":"Rat GNMT activity assays measured SAM-dependent glycine methylation to sarcosine.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"bdebc1d1-ac8b-5954-a2af-ffd3b690b211","mechanism_event_label":"GNMT spends a SAM methyl group on glycine.","subject":{"id":"89d2bf2f-e811-5466-bdb0-f51e12f05845","slug":"gnmt-rat","display_name":"Glycine N-methyltransferase (Rattus norvegicus)","entity_type_key":"protein"},"object":{"id":"14dd468a-0b66-5d2b-b742-7f19acbfb9a5","slug":"sarcosine","display_name":"Sarcosine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"bdebc1d1-ac8b-5954-a2af-ffd3b690b211","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:folate-methyl-gnmt-methyl-use-event","event_type":"biochemical_relationship","label":"GNMT spends a SAM methyl group on glycine.","description":"Rat GNMT activity assays measured SAM-dependent glycine methylation to sarcosine.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"role":"acceptor","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"825f2da2-01bc-5874-a3c6-64f5ac867db5","slug":"s-adenosylmethionine","display_name":"S-Adenosyl-L-methionine","entity_type_key":"small_molecule"},"role":"donor","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"e84829b1-0607-558e-8add-aaa3af4747e7","slug":"s-adenosylhomocysteine","display_name":"S-Adenosyl-L-homocysteine","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"89d2bf2f-e811-5466-bdb0-f51e12f05845","slug":"gnmt-rat","display_name":"Glycine N-methyltransferase (Rattus norvegicus)","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"14dd468a-0b66-5d2b-b742-7f19acbfb9a5","slug":"sarcosine","display_name":"Sarcosine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Native rat liver and bacterially expressed rat GNMT.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Does not quantify whole-body methyl demand.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"folate","display_name":"Folate (vitamin B9)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Rattus norvegicus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"GNMT spends a SAM methyl group on glycine.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[luka-2008] Acetylation of N-terminal valine of glycine N-methyltransferase affects enzyme inhibition by folate (2008). https://pubmed.ncbi.nlm.nih.gov/18501206/ DOI: 10.1016/j.bbapap.2008.04.016","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Native/recombinant rat enzyme","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"147e9903-28ca-5a73-9523-a643d0dc58d3","evidence_kind":"source_excerpt","locator":"Lines 756-765","start_line":756,"end_line":765,"excerpt":"### folate-methyl-gnmt-methyl-use\nRat GNMT activity assays measured SAM-dependent glycine methylation to sarcosine.\nCondition category: normal\nnutrient_topic: Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: GNMT spends a SAM methyl group on glycine.\norganism: Rattus norvegicus\ntissue_or_cell_type: Native/recombinant rat enzyme\nexperimental_model: Native rat liver and bacterially expressed rat GNMT.\nlimitations: Does not quantify whole-body methyl demand.\n[luka-2008] Acetylation of N-terminal valine of glycine N-methyltransferase affects enzyme inhibition by folate (2008). https://pubmed.ncbi.nlm.nih.gov/18501206/ DOI: 10.1016/j.bbapap.2008.04.016","model_system":"Native rat liver and bacterially expressed rat GNMT.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. 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