{"id":"eebca427-80f7-522d-bd0b-e4f10a7fdc0f","stable_key":"1ff3a29f-ef80-5bff-9300-30b98c60b90d:strontium-human-sclerostin","predicate":"reduces","statement":"Strontium exposure decreased sclerostin protein expression in primary human osteoblast cultures in the 2011 study.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"846cc3b3-423a-534f-81e0-98ef5207ecb6","mechanism_event_label":"Removing a Wnt inhibitor can favor a bone-cell response.","subject":{"id":"97607549-3a37-544e-b6e6-266887f7c35f","slug":"strontium-ion","display_name":"Strontium ion / Sr2+","entity_type_key":"ion"},"object":{"id":"9aaf8b7f-26e8-56b1-881f-f323c151598c","slug":"sost","display_name":"Human sclerostin / SOST","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"846cc3b3-423a-534f-81e0-98ef5207ecb6","stable_key":"1ff3a29f-ef80-5bff-9300-30b98c60b90d:strontium-human-sclerostin-event","event_type":"observed_relationship","label":"Removing a Wnt inhibitor can favor a bone-cell response.","description":"Strontium exposure decreased sclerostin protein expression in primary human osteoblast cultures in the 2011 study.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"97607549-3a37-544e-b6e6-266887f7c35f","slug":"strontium-ion","display_name":"Strontium ion / Sr2+","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"9aaf8b7f-26e8-56b1-881f-f323c151598c","slug":"sost","display_name":"Human sclerostin / SOST","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"2ca2864b-1ef0-54e4-8397-1dbe7207a0d2","slug":"strontium","display_name":"Strontium","entity_type_key":"nutrient_element"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human primary osteoblast culture.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Separate from the Akt branch; an earlier long-term culture report found increased SOST mRNA with an osteocyte-like phenotype. Protein, RNA, stage and time are not interchangeable.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Strontium collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"strontium","display_name":"Strontium","entity_type_key":"nutrient_element"}},{"dimension":"plain_language","value_text":"Removing a Wnt inhibitor can favor a bone-cell response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"An Akt-dependent increase in canonical Wnt signaling and a decrease in sclerostin protein levels are involved in strontium ranelate-induced osteogenic effects in human osteoblasts. · 2011 · https://pubmed.ncbi.nlm.nih.gov/21566129/ · DOI 10.1074/jbc.M111.251116","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"7e52d6ea-dc33-57b3-bdd1-88a08a5f256a","evidence_kind":"source_excerpt","locator":"Lines 174-180","start_line":174,"end_line":180,"excerpt":"## strontium-human-sclerostin\nRemoving a Wnt inhibitor can favor a bone-cell response.\nStrontium exposure decreased sclerostin protein expression in primary human osteoblast cultures in the 2011 study.\nModel: Human primary osteoblast culture.\nLimitations: Separate from the Akt branch; an earlier long-term culture report found increased SOST mRNA with an osteocyte-like phenotype. Protein, RNA, stage and time are not interchangeable.\nEvidence access: Primary abstract\nAn Akt-dependent increase in canonical Wnt signaling and a decrease in sclerostin protein levels are involved in strontium ranelate-induced osteogenic effects in human osteoblasts. · 2011 · https://pubmed.ncbi.nlm.nih.gov/21566129/ · DOI 10.1074/jbc.M111.251116","model_system":"Human primary osteoblast culture.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"43ef0fe7-eb6c-5346-8381-f7694733b4dc","stable_key":"import-1ff3a29f-ef80-5bff-9300-30b98c60b90d","title":"Strontium: calcium interactions, cellular mechanisms and mineralization (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"51221949263cdf8a22a79c32e3989599de1a37dc215700e0a076f436b40a1005","revision_id":"84f6df49-c456-5ad2-8214-cc69d5dc7722","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}