{"id":"ee51b5b8-c639-548f-962e-76f879c3f11e","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:fol-uracil-repletion-response","predicate":"supplementation_reduced_dna_uracil","statement":"Blood DNA uracil declined after folic-acid supplementation in study completers, including the initially low-folate subgroup.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"f977d3f2-b82b-56b0-8788-7be9afc71d72","mechanism_event_label":"Restoring folate was followed by improvement in the DNA-base readout.","subject":{"id":"d322bfef-5135-599f-82e6-0aa58748dc8b","slug":"folic-acid","display_name":"Folic acid","entity_type_key":"small_molecule"},"object":{"id":"2bdb9a6d-0a55-59ef-98ce-51168d23d870","slug":"blood-dna-uracil-content","display_name":"Uracil content of blood-cell DNA","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"f977d3f2-b82b-56b0-8788-7be9afc71d72","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:fol-uracil-repletion-response-event","event_type":"observed_intervention","label":"Restoring folate was followed by improvement in the DNA-base readout.","description":"Blood DNA uracil declined after folic-acid supplementation in study completers, including the initially low-folate subgroup.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"d322bfef-5135-599f-82e6-0aa58748dc8b","slug":"folic-acid","display_name":"Folic acid","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2bdb9a6d-0a55-59ef-98ce-51168d23d870","slug":"blood-dna-uracil-content","display_name":"Uracil content of blood-cell DNA","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"experimental_model","value_text":"Selected splenectomized human volunteers: 22 at baseline, 19 supplementation completers; additional blood/marrow samples and Crohn case analyzed separately.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Folic acid 5 mg/day for eight weeks; blood DNA uracil and erythrocyte/reticulocyte micronuclei.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Before/after study with small subgroups and attrition; not a randomized cancer outcome trial.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"folate","display_name":"Folate (vitamin B9)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Restoring folate was followed by improvement in the DNA-base readout.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[fol-blount1997] Folate deficiency causes uracil misincorporation into human DNA and chromosome breakage: implications for cancer and neuronal damage (1997). https://pubmed.ncbi.nlm.nih.gov/9096386/ DOI: 10.1073/pnas.94.7.3290","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Human blood-cell DNA","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"4b051808-c5c9-5153-885b-b7c6f7dbe647","evidence_kind":"source_excerpt","locator":"Lines 1481-1491","start_line":1481,"end_line":1491,"excerpt":"### fol-uracil-repletion-response\nBlood DNA uracil declined after folic-acid supplementation in study completers, including the initially low-folate subgroup.\nCondition category: nutrient_deficiency\nnutrient_topic: Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Restoring folate was followed by improvement in the DNA-base readout.\norganism: Homo sapiens\ntissue_or_cell_type: Human blood-cell DNA\nexperimental_model: Selected splenectomized human volunteers: 22 at baseline, 19 supplementation completers; additional blood/marrow samples and Crohn case analyzed separately.\nlimitations: Before/after study with small subgroups and attrition; not a randomized cancer outcome trial.\nexposure: Folic acid 5 mg/day for eight weeks; blood DNA uracil and erythrocyte/reticulocyte micronuclei.\n[fol-blount1997] Folate deficiency causes uracil misincorporation into human DNA and chromosome breakage: implications for cancer and neuronal damage (1997). https://pubmed.ncbi.nlm.nih.gov/9096386/ DOI: 10.1073/pnas.94.7.3290","model_system":"Selected splenectomized human volunteers: 22 at baseline, 19 supplementation completers; additional blood/marrow samples and Crohn case analyzed separately.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [fol-blount1997] Folate deficiency causes uracil misincorporation into human DNA and chromosome breakage: implications for cancer and neuronal damage (1997). https://pubmed.ncbi.nlm.nih.gov/9096386/ DOI: 10.1073/pnas.94.7.3290","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"f4ce1a62-9582-5f7a-84f5-a23d0e1bfc68","stable_key":"import-ec174d5a-4903-5745-8646-df0e9d4265e8","title":"Folate and folic acid: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e564d43989ece1006c95cd0748e9af6fe369074599a2eebba0a99ebff864b0dd","revision_id":"76674a33-b2a1-5e41-b71b-44399038ff7c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}