{"id":"ee48262b-882b-5e50-9a45-b20d55c15d96","stable_key":"fd37d270-3395-56d6-9f1c-ddda56e606f6:arg-amd-feedback","predicate":"activates","statement":"Putrescine stimulated human AMD1 autoprocessing and decarboxylation through a binding pocket separate from the active site.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"c29a80dc-d78a-57b4-8f1c-bc180734afb7","mechanism_event_label":"A pathway product helps activate production of the next branch’s donor.","subject":{"id":"c71bf622-f14b-5314-b1a5-dfa002d2d8ff","slug":"putrescine","display_name":"Putrescine","entity_type_key":"small_molecule"},"object":{"id":"19cac4f8-8921-5216-8ee1-c70e6a1220d7","slug":"amd1","display_name":"Human S-adenosylmethionine decarboxylase / AMD1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"c29a80dc-d78a-57b4-8f1c-bc180734afb7","stable_key":"fd37d270-3395-56d6-9f1c-ddda56e606f6:arg-amd-feedback-event","event_type":"observed_relationship","label":"A pathway product helps activate production of the next branch’s donor.","description":"Putrescine stimulated human AMD1 autoprocessing and decarboxylation through a binding pocket separate from the active site.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"c71bf622-f14b-5314-b1a5-dfa002d2d8ff","slug":"putrescine","display_name":"Putrescine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"19cac4f8-8921-5216-8ee1-c70e6a1220d7","slug":"amd1","display_name":"Human S-adenosylmethionine decarboxylase / AMD1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"825f2da2-01bc-5874-a3c6-64f5ac867db5","slug":"s-adenosylmethionine","display_name":"S-Adenosyl-L-methionine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"ae5a8bbb-59e5-503a-94c9-51b512d7c7b3","slug":"decarboxylated-sam","display_name":"Decarboxylated S-adenosylmethionine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human AMD1 structures, binding measurements and targeted mutants.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Feedback demonstrated at enzyme level; not a measured systemic methyl-donor drain.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Arginine collection; tissue, species, dose and formulation distinctions retained.","comparator":null,"unit":null,"notes":"","entity":{"slug":"arginine","display_name":"L-Arginine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A pathway product helps activate production of the next branch’s donor.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Structural basis for putrescine activation of human S-adenosylmethionine decarboxylase. · 2008 · https://pubmed.ncbi.nlm.nih.gov/19053272/ · DOI 10.1021/bi801732m","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"05cf3f27-7b1b-520c-bd20-3cf40ac813a9","evidence_kind":"source_excerpt","locator":"Lines 142-148","start_line":142,"end_line":148,"excerpt":"## arg-amd-feedback\nA pathway product helps activate production of the next branch’s donor.\nPutrescine stimulated human AMD1 autoprocessing and decarboxylation through a binding pocket separate from the active site.\nModel: Human AMD1 structures, binding measurements and targeted mutants.\nLimitations: Feedback demonstrated at enzyme level; not a measured systemic methyl-donor drain.\nEvidence access: Primary abstract\nStructural basis for putrescine activation of human S-adenosylmethionine decarboxylase. · 2008 · https://pubmed.ncbi.nlm.nih.gov/19053272/ · DOI 10.1021/bi801732m","model_system":"Human AMD1 structures, binding measurements and targeted mutants.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; primary evidence access stated per claim.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"bb9a67ea-904f-5b8a-93a4-459081212bdd","stable_key":"import-fd37d270-3395-56d6-9f1c-ddda56e606f6","title":"L-Arginine: transport, metabolic branches, nutrient interactions, availability and discovery questions (2026-09-18)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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