{"id":"ee0bcf38-3e33-50d1-824d-50f0c26a9329","stable_key":"6d38d43e-01e4-5641-93be-65654271e242:zinc-trans-zip4-repletion-endocytosis","predicate":"stimulates","statement":"Oral zinc repletion caused mouse ZIP4 internalization from enterocyte apical membranes after dietary zinc deficiency.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"4d5aaaaf-50d4-53ff-8481-0cde97e27589","mechanism_event_label":"After zinc becomes available again, intestinal cells pull ZIP4 away from their surface.","subject":{"id":"49c806c2-7041-5020-8b3b-fa04ffa122ac","slug":"zinc-ion","display_name":"Zinc(II) ion","entity_type_key":"ion"},"object":{"id":"1e5676c5-592c-5b8a-9ac1-77c34ee142dd","slug":"zip4-endocytosis","display_name":"ZIP4 endocytosis","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"4d5aaaaf-50d4-53ff-8481-0cde97e27589","stable_key":"6d38d43e-01e4-5641-93be-65654271e242:zinc-trans-zip4-repletion-endocytosis-event","event_type":"biochemical_relationship","label":"After zinc becomes available again, intestinal cells pull ZIP4 away from their surface.","description":"Oral zinc repletion caused mouse ZIP4 internalization from enterocyte apical membranes after dietary zinc deficiency.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b2399154-5b80-5772-bf7c-d9d80aefb691","slug":"mouse-slc39a4","display_name":"Mouse ZIP4 (Slc39a4)","entity_type_key":"protein"},"role":"internalized_transporter","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"49c806c2-7041-5020-8b3b-fa04ffa122ac","slug":"zinc-ion","display_name":"Zinc(II) ion","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"1e5676c5-592c-5b8a-9ac1-77c34ee142dd","slug":"zip4-endocytosis","display_name":"ZIP4 endocytosis","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Dietary depletion followed by oral gavage and immunolocalization","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Zinc-deficient weanlings for 10 days; 100 micromol ZnCl2/kg oral gavage with time-course localization.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Acute experimental repletion; the gavage is not a recommended human dose and internalization is distinct from later degradation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Zinc research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"zinc","display_name":"Zinc","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"After zinc becomes available again, intestinal cells pull ZIP4 away from their surface.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[zinc-trans-18020946] Novel zinc-responsive post-transcriptional mechanisms reciprocally regulate expression of the mouse Slc39a4 and Slc39a5 zinc transporters (Zip4 and Zip5). (2007). https://pubmed.ncbi.nlm.nih.gov/18020946/ DOI: 10.1515/bc.2007.149","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Duodenal enterocytes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"c8143c65-c845-58f2-a2e6-3d71a15468f6","evidence_kind":"source_excerpt","locator":"Lines 245-256","start_line":245,"end_line":256,"excerpt":"### zinc-trans-zip4-repletion-endocytosis\nOral zinc repletion caused mouse ZIP4 internalization from enterocyte apical membranes after dietary zinc deficiency.\nCondition category: nutrient_deficiency\nnutrient_topic: Zinc research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: After zinc becomes available again, intestinal cells pull ZIP4 away from their surface.\norganism: Mus musculus\ntissue_or_cell_type: Duodenal enterocytes\nexperimental_model: Dietary depletion followed by oral gavage and immunolocalization\nlimitations: Acute experimental repletion; the gavage is not a recommended human dose and internalization is distinct from later degradation.\nexposure: Zinc-deficient weanlings for 10 days; 100 micromol ZnCl2/kg oral gavage with time-course localization.\ncross_nutrient: false\n[zinc-trans-18020946] Novel zinc-responsive post-transcriptional mechanisms reciprocally regulate expression of the mouse Slc39a4 and Slc39a5 zinc transporters (Zip4 and Zip5). (2007). https://pubmed.ncbi.nlm.nih.gov/18020946/ DOI: 10.1515/bc.2007.149","model_system":"Dietary depletion followed by oral gavage and immunolocalization","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [zinc-trans-18020946] Novel zinc-responsive post-transcriptional mechanisms reciprocally regulate expression of the mouse Slc39a4 and Slc39a5 zinc transporters (Zip4 and Zip5). (2007). https://pubmed.ncbi.nlm.nih.gov/18020946/ DOI: 10.1515/bc.2007.149","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"c5ee0fee-ce5c-58de-905a-10fb0ea0723c","stable_key":"import-6d38d43e-01e4-5641-93be-65654271e242","title":"Zinc: transport, enzyme loading, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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