{"id":"eddfc4c2-6254-5995-b0c1-1ff05d09cbf4","stable_key":"6cdb37aa-8998-5ea8-829d-4f995caf98fc:l-carnitine-human-crat-reaction","predicate":"forms","statement":"Purified recombinant human CRAT transferred short-chain acyl groups including acetyl from acyl-CoA substrates to carnitine in enzyme assays.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"df9353e8-9a4b-538d-bfc4-2c36f364e031","mechanism_event_label":"The human enzyme directly connects carnitine and the CoA pool.","subject":{"id":"507b3d2c-db75-51d1-823a-0677e0c1a0e8","slug":"human-crat","display_name":"Human carnitine acetyltransferase / CRAT","entity_type_key":"protein"},"object":{"id":"5ce394a9-6ec6-56dd-866f-37d85c0556e4","slug":"acetylcarnitine","display_name":"Acetyl-L-carnitine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"df9353e8-9a4b-538d-bfc4-2c36f364e031","stable_key":"6cdb37aa-8998-5ea8-829d-4f995caf98fc:l-carnitine-human-crat-reaction-event","event_type":"observed_relationship","label":"The human enzyme directly connects carnitine and the CoA pool.","description":"Purified recombinant human CRAT transferred short-chain acyl groups including acetyl from acyl-CoA substrates to carnitine in enzyme assays.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"507b3d2c-db75-51d1-823a-0677e0c1a0e8","slug":"human-crat","display_name":"Human carnitine acetyltransferase / CRAT","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"5ce394a9-6ec6-56dd-866f-37d85c0556e4","slug":"acetylcarnitine","display_name":"Acetyl-L-carnitine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"b7ed6e4c-e560-5cee-b68f-895f21862e6f","slug":"acetyl-coa","display_name":"Acetyl-CoA","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"4faa6456-aff8-59eb-9e3f-3326a436e401","slug":"coenzyme-a","display_name":"Coenzyme A","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"6e34c035-9371-578f-b799-fd2a14e9e40f","slug":"l-carnitine","display_name":"L-Carnitine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full-text Table 1 and recombinant enzyme methods","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human CRAT expressed in E. coli; steady-state kinetic substrate panel.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Purified-enzyme capacity does not identify the dominant flux in every compartment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Carnitine collection; isomer, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-carnitine","display_name":"L-Carnitine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The human enzyme directly connects carnitine and the CoA pool.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Carnitine octanoyltransferase is important for the assimilation of exogenous acetyl-L-carnitine into acetyl-CoA in mammalian cells. · 2023 · https://pubmed.ncbi.nlm.nih.gov/36587768/ · DOI 10.1016/j.jbc.2022.102848","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"79b606c5-c304-53d5-9178-b4f90cfb0918","evidence_kind":"source_excerpt","locator":"Lines 498-504","start_line":498,"end_line":504,"excerpt":"## l-carnitine-human-crat-reaction\nThe human enzyme directly connects carnitine and the CoA pool.\nPurified recombinant human CRAT transferred short-chain acyl groups including acetyl from acyl-CoA substrates to carnitine in enzyme assays.\nModel: Human CRAT expressed in E. coli; steady-state kinetic substrate panel.\nLimitations: Purified-enzyme capacity does not identify the dominant flux in every compartment.\nEvidence access: Primary full-text Table 1 and recombinant enzyme methods\nCarnitine octanoyltransferase is important for the assimilation of exogenous acetyl-L-carnitine into acetyl-CoA in mammalian cells. · 2023 · https://pubmed.ncbi.nlm.nih.gov/36587768/ · DOI 10.1016/j.jbc.2022.102848","model_system":"Human CRAT expressed in E. coli; steady-state kinetic substrate panel.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e2c9c42b-806a-55e2-abde-b0320ea94704","stable_key":"import-6cdb37aa-8998-5ea8-829d-4f995caf98fc","title":"L-Carnitine: synthesis, acyl-group transport, fuel selection and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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