{"id":"eddabf58-ba5d-55f2-b6e0-67271d7068ab","stable_key":"1fb6218a-60d1-55ea-87fe-07d15d0fc46f:statin-inhibits-rho-membrane-translocation","predicate":"reduces","statement":"Mevastatin inhibited Rho membrane translocation by about 60 percent and Rho GTP-binding activity by about 78 percent, both reversed by geranylgeranyl diphosphate but not by farnesyl diphosphate.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"d950d1eb-44ad-5681-9cbd-381a34387062","mechanism_event_label":"Mevastatin inhibited Rho membrane translocation by about 60 percent and Rho GTP-binding activity by about 78 percent, both reversed by geranylgeranyl diphosphate but not by farnesyl diphosphate.","subject":{"id":"aa3152b9-b04f-5a0d-bf87-e9c1dbe0e1f9","slug":"mevastatin","display_name":"Mevastatin","entity_type_key":"small_molecule"},"object":{"id":"1685620f-5bc5-50df-8342-00e87365a515","slug":"rho-membrane-translocation","display_name":"Rho membrane translocation and GTP binding","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"d950d1eb-44ad-5681-9cbd-381a34387062","stable_key":"1fb6218a-60d1-55ea-87fe-07d15d0fc46f:statin-inhibits-rho-membrane-translocation-event","event_type":"observed_relationship","label":"Mevastatin inhibited Rho membrane translocation by about 60 percent and Rho GTP-binding activity by about 78 percent, both reversed by geranylgeranyl diphosphate but not by farnesyl diphosphate.","description":"Mevastatin inhibited Rho membrane translocation by about 60 percent and Rho GTP-binding activity by about 78 percent, both reversed by geranylgeranyl diphosphate but not by farnesyl diphosphate.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"aa3152b9-b04f-5a0d-bf87-e9c1dbe0e1f9","slug":"mevastatin","display_name":"Mevastatin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"1685620f-5bc5-50df-8342-00e87365a515","slug":"rho-membrane-translocation","display_name":"Rho membrane translocation and GTP binding","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7e6d85c1-25b7-549b-9883-8e9d5d11ae3d","slug":"geranylgeranyl-diphosphate","display_name":"Geranylgeranyl diphosphate / GGPP","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"9ccdf853-7005-5a53-b053-83574a870ad7","slug":"farnesyl-diphosphate","display_name":"Farnesyl diphosphate / FPP","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"duration","value_text":"Not stated here","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human endothelial cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Mevastatin with or without geranylgeranyl diphosphate or farnesyl diphosphate","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Measured for Rho as a group by immunoblot and GTP-gamma-S binding, not resolved to individual Rho family members.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Human endothelial cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Mevastatin inhibited Rho membrane translocation by about 60 percent and Rho GTP-binding activity by about 78 percent, both reversed by geranylgeranyl diphosphate but not by farnesyl diphosphate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Post-transcriptional regulation of endothelial nitric oxide synthase mRNA stability by Rho GTPase. (1998). https://pubmed.ncbi.nlm.nih.gov/9727051/ DOI: 10.1074/jbc.273.37.24266","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Rho GTPase membrane localisation and nucleotide binding","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"6201c128-780a-5b55-ab33-afb6182a98a3","evidence_kind":"source_excerpt","locator":"Lines 78-87","start_line":78,"end_line":87,"excerpt":"## statin-inhibits-rho-membrane-translocation\nMevastatin inhibited Rho membrane translocation by about 60 percent and Rho GTP-binding activity by about 78 percent, both reversed by geranylgeranyl diphosphate but not by farnesyl diphosphate.\nModel/species: Human endothelial cells\nTissue/system: Rho GTPase membrane localisation and nucleotide binding\nExposure: Mevastatin with or without geranylgeranyl diphosphate or farnesyl diphosphate\nRoute: In vitro\nDuration: Not stated here\nLimits: Measured for Rho as a group by immunoblot and GTP-gamma-S binding, not resolved to individual Rho family members.\nPrimary reference: Post-transcriptional regulation of endothelial nitric oxide synthase mRNA stability by Rho GTPase. (1998). https://pubmed.ncbi.nlm.nih.gov/9727051/ DOI: 10.1074/jbc.273.37.24266\nAccess: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","model_system":"","directness":"reported_statement","verification_status":"source_derived_draft","notes":"","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"a4431d8c-34d7-5db7-9edf-a35f68feba39","stable_key":"import-1fb6218a-60d1-55ea-87fe-07d15d0fc46f","title":"Atorvastatin: mechanism of action from target occupancy to isoprenoids, transport, muscle and metabolism (2026-09-22)","document_type":"imported_text","citation_label":"Original AI-assisted curation of twelve primary studies resolved by PubMed title search and cross-checked against live PubMed metadata. Findings obtained with mevastatin, simvastatin or the statin class are recorded against those subjects. Study-specific citations, doses, negative findings and limitations retained. Not publisher full text.","file_path":"","sha256":"6307f1acf99f90662320328974781ee660348754257b1e1555701ad597edc8e5","revision_id":"447c5978-ba3f-58fe-ac85-664caa72e01b","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}