{"id":"edd804bb-7994-5933-8b10-a5c2d4d4f185","stable_key":"88eb7407-f147-558b-889b-0c89c4e0aa4a:spermidine-ep300-loss","predicate":"knockdown_increases","statement":"EP300 knockdown induced autophagy and reduced mTORC1 activity in the acetyltransferase screen.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"ff7d6a3e-e6da-50bc-b297-cdd158db4aba","mechanism_event_label":"Removing a regulator supports a causal pathway.","subject":{"id":"9052514f-cb56-57bc-8df8-62b26b2ce3a2","slug":"ep300","display_name":"EP300","entity_type_key":"protein"},"object":{"id":"63c6f515-f270-577c-ae5a-742fa5a00309","slug":"human-cell-autophagic-flux","display_name":"Human cultured-cell autophagic flux","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"ff7d6a3e-e6da-50bc-b297-cdd158db4aba","stable_key":"88eb7407-f147-558b-889b-0c89c4e0aa4a:spermidine-ep300-loss-event","event_type":"observed_relationship","label":"Removing a regulator supports a causal pathway.","description":"EP300 knockdown induced autophagy and reduced mTORC1 activity in the acetyltransferase screen.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"9052514f-cb56-57bc-8df8-62b26b2ce3a2","slug":"ep300","display_name":"EP300","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"63c6f515-f270-577c-ae5a-742fa5a00309","slug":"human-cell-autophagic-flux","display_name":"Human cultured-cell autophagic flux","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7014993c-468f-5c6b-ab04-80c87f9dfd9f","slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human-cell RNA interference screen of 43 acetyltransferases.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Does not prove all spermidine effects are mediated by EP300.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Spermidine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Removing a regulator supports a causal pathway.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Spermidine induces autophagy by inhibiting the acetyltransferase EP300. · 2015 · https://pubmed.ncbi.nlm.nih.gov/25526088/ · DOI 10.1038/cdd.2014.215","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"eef4a9d9-81bb-5650-83cf-57816aa6fd19","evidence_kind":"source_excerpt","locator":"Lines 174-180","start_line":174,"end_line":180,"excerpt":"## spermidine-ep300-loss\nRemoving a regulator supports a causal pathway.\nEP300 knockdown induced autophagy and reduced mTORC1 activity in the acetyltransferase screen.\nModel: Human-cell RNA interference screen of 43 acetyltransferases.\nLimitations: Does not prove all spermidine effects are mediated by EP300.\nEvidence access: Primary abstract\nSpermidine induces autophagy by inhibiting the acetyltransferase EP300. · 2015 · https://pubmed.ncbi.nlm.nih.gov/25526088/ · DOI 10.1038/cdd.2014.215","model_system":"Human-cell RNA interference screen of 43 acetyltransferases.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4348f3f8-0180-58a3-b61c-55d03bb322d2","stable_key":"import-88eb7407-f147-558b-889b-0c89c4e0aa4a","title":"Spermidine: biosynthesis, hypusination, transport and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"8eec8784bc2bd0384cfd53c6603c4ced8e53c227d9ab29ed85594c43e34c68ef","revision_id":"7c421a18-4526-59f6-b84e-4be533bc35ac","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}