{"id":"edc72da8-042c-5320-98de-62d71283e8b8","stable_key":"335be270-ea4a-5c8e-ad04-964fd22a439e:vanadium-bone-no","predicate":"increases","statement":"Vanadate increased NO release above 50 micromolar in the tested rat UMR106 and mouse MC3T3-E1 osteoblast-like cultures, with a biphasic concentration response.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"7b4f3c38-6d9c-5b49-96ec-c215e3ee80a8","mechanism_event_label":"A growth-related exposure can engage nitrosative signaling at higher concentrations.","subject":{"id":"bef86a6e-28a0-5df9-9306-3f05991aa159","slug":"vanadate-v","display_name":"Vanadate(V), protonation/speciation dependent","entity_type_key":"chemical_species"},"object":{"id":"516e7fc8-b1a4-527b-8087-1262c9db94bd","slug":"vanadate-osteoblast-nitric-oxide","display_name":"NO production in vanadate-exposed osteoblast-like cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"7b4f3c38-6d9c-5b49-96ec-c215e3ee80a8","stable_key":"335be270-ea4a-5c8e-ad04-964fd22a439e:vanadium-bone-no-event","event_type":"observed_relationship","label":"A growth-related exposure can engage nitrosative signaling at higher concentrations.","description":"Vanadate increased NO release above 50 micromolar in the tested rat UMR106 and mouse MC3T3-E1 osteoblast-like cultures, with a biphasic concentration response.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"bef86a6e-28a0-5df9-9306-3f05991aa159","slug":"vanadate-v","display_name":"Vanadate(V), protonation/speciation dependent","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"516e7fc8-b1a4-527b-8087-1262c9db94bd","slug":"vanadate-osteoblast-nitric-oxide","display_name":"NO production in vanadate-exposed osteoblast-like cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c5cbad45-22c6-594b-ab6b-71880dab1b24","slug":"vanadium","display_name":"Vanadium","entity_type_key":"nutrient_element"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"f2a6b7ab-ad41-5ada-8895-41d6ce42f4e4","slug":"nitric-oxide","display_name":"NO","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rodent osteoblast-like cell cultures; 2.5–100 micromolar vanadate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Mixed cell models are named explicitly; NO is a proposed mediator, not proven sole cause of growth effects.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vanadium collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vanadium","display_name":"Vanadium","entity_type_key":"nutrient_element"}},{"dimension":"plain_language","value_text":"A growth-related exposure can engage nitrosative signaling at higher concentrations.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Vanadate-induced nitric oxide production: role in osteoblast growth and differentiation. · 2000 · https://pubmed.ncbi.nlm.nih.gov/10988345/ · DOI 10.1016/s0014-2999(00)00356-3","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"9d96b4c0-cf78-51a9-8b1d-0554f618193f","evidence_kind":"source_excerpt","locator":"Lines 326-332","start_line":326,"end_line":332,"excerpt":"## vanadium-bone-no\nA growth-related exposure can engage nitrosative signaling at higher concentrations.\nVanadate increased NO release above 50 micromolar in the tested rat UMR106 and mouse MC3T3-E1 osteoblast-like cultures, with a biphasic concentration response.\nModel: Rodent osteoblast-like cell cultures; 2.5–100 micromolar vanadate.\nLimitations: Mixed cell models are named explicitly; NO is a proposed mediator, not proven sole cause of growth effects.\nEvidence access: Primary abstract\nVanadate-induced nitric oxide production: role in osteoblast growth and differentiation. · 2000 · https://pubmed.ncbi.nlm.nih.gov/10988345/ · DOI 10.1016/s0014-2999(00)00356-3","model_system":"Rodent osteoblast-like cell cultures; 2.5–100 micromolar vanadate.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e4d5d0ba-529b-540f-96ed-e719d116ccfd","stable_key":"import-335be270-ea4a-5c8e-ad04-964fd22a439e","title":"Vanadium: speciation, phosphate-sensitive enzymes and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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