{"id":"ed6060b8-1897-56c9-bddd-db3cfb1bf125","stable_key":"c932089b-ebc9-5f39-b2e5-84af74f9f6d0:sucrose-fluoride-mineral","predicate":"reduces","statement":"Increasing fluoride from 0.50 to 0.75 ppm reduced enamel mineral loss during repeated sucrose exposure in the artificial-mouth model.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"7c0efcba-d20b-5613-a442-94c9bca761f5","mechanism_event_label":"Increasing fluoride from 0.50 to 0.75 ppm reduced enamel mineral loss during repeated sucrose exposure in the artificial-mouth model.","subject":{"id":"ea5d512c-d972-5d33-8dcc-f4b158b5c2fb","slug":"fluoride-ion","display_name":"Fluoride ion","entity_type_key":"ion"},"object":{"id":"fbf7bb98-272f-5fc5-935e-ca120bf16975","slug":"human-enamel-artificial-mouth-mineral-loss","display_name":"Mineral loss from human enamel in artificial-mouth biofilm experiments","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"7c0efcba-d20b-5613-a442-94c9bca761f5","stable_key":"c932089b-ebc9-5f39-b2e5-84af74f9f6d0:sucrose-fluoride-mineral-event","event_type":"observed_relationship","label":"Increasing fluoride from 0.50 to 0.75 ppm reduced enamel mineral loss during repeated sucrose exposure in the artificial-mouth model.","description":"Increasing fluoride from 0.50 to 0.75 ppm reduced enamel mineral loss during repeated sucrose exposure in the artificial-mouth model.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ea5d512c-d972-5d33-8dcc-f4b158b5c2fb","slug":"fluoride-ion","display_name":"Fluoride ion","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"fbf7bb98-272f-5fc5-935e-ca120bf16975","slug":"human-enamel-artificial-mouth-mineral-loss","display_name":"Mineral loss from human enamel in artificial-mouth biofilm experiments","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"82558bd0-1a84-574a-a04c-10609c9c5dba","slug":"sucrose","display_name":"Sucrose","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"3% or 5% sucrose for 30 minutes three times/day; 0.50 or 0.75 ppm fluoride for 22.5 hours/day","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"5 days; separate 3-versus-9-day biofilm comparison","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary full-text methods/results and metadata inspected.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; source-specific curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human enamel slabs with mixed streptococcal/lactobacillus biofilms in artificial mouth","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure_scope","value_text":"Sucrose, bacterial biofilm and fluoride interaction","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Artificial-mouth lesion endpoint, not systemic fluoride or clinical prevention estimate. 3 versus 5% sucrose did not differ under this protocol; this does not establish general dose independence.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Sucrose chapter; direct sucrose observations are distinguished from shared component metabolism.","comparator":null,"unit":null,"notes":"","entity":{"slug":"sucrose","display_name":"Sucrose","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human enamel slabs with mixed streptococcal/lactobacillus biofilms in artificial mouth","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Increasing fluoride from 0.50 to 0.75 ppm reduced enamel mineral loss during repeated sucrose exposure in the artificial-mouth model.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Enamel Carious Lesion Development in Response to Sucrose and Fluoride Concentrations and to Time of Biofilm Formation: An Artificial-Mouth Study. (2014). https://pubmed.ncbi.nlm.nih.gov/25664342/ DOI: 10.1155/2014/348032","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro repeated local exposure","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Enamel lesion depth and integrated mineral loss","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"8e8717b6-1510-5c58-b2d3-bac71d3c24ca","evidence_kind":"source_excerpt","locator":"Lines 463-473","start_line":463,"end_line":473,"excerpt":"## sucrose-fluoride-mineral\nIncreasing fluoride from 0.50 to 0.75 ppm reduced enamel mineral loss during repeated sucrose exposure in the artificial-mouth model.\nModel/species: Human enamel slabs with mixed streptococcal/lactobacillus biofilms in artificial mouth\nTissue: Enamel lesion depth and integrated mineral loss\nExposure: 3% or 5% sucrose for 30 minutes three times/day; 0.50 or 0.75 ppm fluoride for 22.5 hours/day\nRoute: In vitro repeated local exposure\nDuration: 5 days; separate 3-versus-9-day biofilm comparison\nExposure scope: Sucrose, bacterial biofilm and fluoride interaction\nLimits: Artificial-mouth lesion endpoint, not systemic fluoride or clinical prevention estimate. 3 versus 5% sucrose did not differ under this protocol; this does not establish general dose independence.\nReference: Enamel Carious Lesion Development in Response to Sucrose and Fluoride Concentrations and to Time of Biofilm Formation: An Artificial-Mouth Study. (2014). https://pubmed.ncbi.nlm.nih.gov/25664342/ DOI: 10.1155/2014/348032\nAccess: Primary full-text methods/results and metadata inspected.","model_system":"Human enamel slabs with mixed streptococcal/lactobacillus biofilms in artificial mouth","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span, not a publisher quotation; provenance retained.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"8e24ddf4-84ee-57b4-9fd8-093547432ece","stable_key":"import-c932089b-ebc9-5f39-b2e5-84af74f9f6d0","title":"Sucrose: mechanism of action and metabolic impact (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted source-specific sucrose curation with shared canonical claims retained by identity. Primary-study citations, negative findings, exposure details and limitations preserved. 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