{"id":"ecfa5707-2f56-5ef9-8176-1722d21d23cf","stable_key":"911fb3c7-8cc3-5667-b677-5682fab67648:histidine-pht1-brain-uptake","predicate":"supports","statement":"Deleting Pht1 reduced histidine uptake in mouse brain slices by about half; Pept2 expression increased approximately twofold.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"60e112f6-331a-5c3a-9bf9-c0851df27daa","mechanism_event_label":"Loss of one transporter altered uptake even with a compensatory response.","subject":{"id":"6ec79306-1983-566c-8dd0-b100a18afc9e","slug":"mouse-slc15a4","display_name":"Mouse peptide/histidine transporter Pht1 / Slc15a4","entity_type_key":"protein"},"object":{"id":"a94d61a0-e36e-5424-9cea-be422750871b","slug":"mouse-brain-histidine-uptake","display_name":"Histidine uptake into mouse brain slices","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"60e112f6-331a-5c3a-9bf9-c0851df27daa","stable_key":"911fb3c7-8cc3-5667-b677-5682fab67648:histidine-pht1-brain-uptake-event","event_type":"observed_relationship","label":"Loss of one transporter altered uptake even with a compensatory response.","description":"Deleting Pht1 reduced histidine uptake in mouse brain slices by about half; 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unverified.","entity":null}],"evidence":[{"id":"a0f0d7d7-91e4-5dfd-8b7a-9231e89c9e1d","evidence_kind":"source_excerpt","locator":"Lines 90-96","start_line":90,"end_line":96,"excerpt":"## histidine-pht1-brain-uptake\nLoss of one transporter altered uptake even with a compensatory response.\nDeleting Pht1 reduced histidine uptake in mouse brain slices by about half; Pept2 expression increased approximately twofold.\nModel: Pht1-null and matched wild-type adult mice; brain slices and transporter expression.\nLimitations: Genetic deletion is not dietary histidine deficiency.\nEvidence access: Primary full text\nA novel role for PHT1 in the disposition of l-histidine in brain: In vitro slice and in vivo pharmacokinetic studies in wildtype and Pht1 null mice. · 2017 · https://pubmed.ncbi.nlm.nih.gov/27845049/ · DOI 10.1016/j.bcp.2016.11.012","model_system":"Pht1-null and matched wild-type adult mice; brain slices and transporter expression.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; 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