{"id":"ecf1c44f-a52a-50db-aca4-f6700e8f07b7","stable_key":"1ff3a29f-ef80-5bff-9300-30b98c60b90d:strontium-keratinocyte-differentiation","predicate":"does_not_replace_calcium_for","statement":"Strontium-supported keratinocyte cultures lacked the stratification and desmosomes found with 1.8 mM calcium, despite increased cell yield.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"b6f88dc8-9ced-513c-9734-08d727f6974a","mechanism_event_label":"Supporting cell growth did not replace calcium’s differentiation function.","subject":{"id":"98c86880-8291-5dae-9d2d-1b33bea19815","slug":"strontium-chloride","display_name":"Strontium chloride","entity_type_key":"chemical_species"},"object":{"id":"5a5283a7-3198-5e32-8783-d69981a87e43","slug":"human-keratinocyte-terminal-differentiation","display_name":"Terminal differentiation and desmosomes in human keratinocytes","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"b6f88dc8-9ced-513c-9734-08d727f6974a","stable_key":"1ff3a29f-ef80-5bff-9300-30b98c60b90d:strontium-keratinocyte-differentiation-event","event_type":"observed_relationship","label":"Supporting cell growth did not replace calcium’s differentiation function.","description":"Strontium-supported keratinocyte cultures lacked the stratification and desmosomes found with 1.8 mM calcium, despite increased cell yield.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"98c86880-8291-5dae-9d2d-1b33bea19815","slug":"strontium-chloride","display_name":"Strontium chloride","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"5a5283a7-3198-5e32-8783-d69981a87e43","slug":"human-keratinocyte-terminal-differentiation","display_name":"Terminal differentiation and desmosomes in human keratinocytes","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"2ca2864b-1ef0-54e4-8397-1dbe7207a0d2","slug":"strontium","display_name":"Strontium","entity_type_key":"nutrient_element"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Electron microscopy and morphology in human keratinocyte cultures.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This does not define whole-body calcium deficiency or a dermatologic therapy.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Strontium collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"strontium","display_name":"Strontium","entity_type_key":"nutrient_element"}},{"dimension":"plain_language","value_text":"Supporting cell growth did not replace calcium’s differentiation function.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Use of strontium to separate calcium-dependent pathways for proliferation and differentiation in human keratinocytes. · 1987 · https://pubmed.ncbi.nlm.nih.gov/2439523/ · DOI 10.1002/jcp.1041320111","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"d371ae6b-55fc-5811-9956-8a27e403165e","evidence_kind":"source_excerpt","locator":"Lines 414-420","start_line":414,"end_line":420,"excerpt":"## strontium-keratinocyte-differentiation\nSupporting cell growth did not replace calcium’s differentiation function.\nStrontium-supported keratinocyte cultures lacked the stratification and desmosomes found with 1.8 mM calcium, despite increased cell yield.\nModel: Electron microscopy and morphology in human keratinocyte cultures.\nLimitations: This does not define whole-body calcium deficiency or a dermatologic therapy.\nEvidence access: Primary abstract\nUse of strontium to separate calcium-dependent pathways for proliferation and differentiation in human keratinocytes. · 1987 · https://pubmed.ncbi.nlm.nih.gov/2439523/ · DOI 10.1002/jcp.1041320111","model_system":"Electron microscopy and morphology in human keratinocyte cultures.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"43ef0fe7-eb6c-5346-8381-f7694733b4dc","stable_key":"import-1ff3a29f-ef80-5bff-9300-30b98c60b90d","title":"Strontium: calcium interactions, cellular mechanisms and mineralization (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"51221949263cdf8a22a79c32e3989599de1a37dc215700e0a076f436b40a1005","revision_id":"84f6df49-c456-5ad2-8214-cc69d5dc7722","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}