{"id":"eceadde5-2868-598c-9412-985124103da8","stable_key":"507ed066-fd6d-5722-9223-97b66302e7e3:glycine-gpr158-potassium-current","predicate":"reduces_tested","statement":"In mouse accumbens slices, 1 mM glycine increased medium-spiny-neuron firing and reduced M-current amplitude; a selective M-current inhibitor mimicked and occluded the response.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"87ea6d01-ac51-5bb2-b5a3-e888b902a768","mechanism_event_label":"Glycine-sensitive signaling intersects with potassium-channel control of firing.","subject":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"object":{"id":"0df8aee0-4873-5640-a11b-d1c5dff92758","slug":"mouse-nac-m-current","display_name":"Potassium M-current in mouse accumbens medium spiny neurons","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"87ea6d01-ac51-5bb2-b5a3-e888b902a768","stable_key":"507ed066-fd6d-5722-9223-97b66302e7e3:glycine-gpr158-potassium-current-event","event_type":"observed_relationship","label":"Glycine-sensitive signaling intersects with potassium-channel control of firing.","description":"In mouse accumbens slices, 1 mM glycine increased medium-spiny-neuron firing and reduced M-current amplitude; 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slice patch clamp with synaptic blockers.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Authors attributed the response to GPR158; pharmacology alone is weaker than a receptor-knockout test and does not prove direct channel binding.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Glycine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Glycine-sensitive signaling intersects with potassium-channel control of firing.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Glycine-induced activation of GPR158 increases the intrinsic excitability of medium spiny neurons in the nucleus accumbens. · 2024 · https://pubmed.ncbi.nlm.nih.gov/38884814/ · DOI 10.1007/s00018-024-05260-w","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"9817d299-00e8-5d81-ad1e-2c5f30195d28","evidence_kind":"source_excerpt","locator":"Lines 258-264","start_line":258,"end_line":264,"excerpt":"## glycine-gpr158-potassium-current\nGlycine-sensitive signaling intersects with potassium-channel control of firing.\nIn mouse accumbens slices, 1 mM glycine increased medium-spiny-neuron firing and reduced M-current amplitude; a selective M-current inhibitor mimicked and occluded the response.\nModel: Male C57BL/6J mice aged four to six weeks; slice patch clamp with synaptic blockers.\nLimitations: Authors attributed the response to GPR158; pharmacology alone is weaker than a receptor-knockout test and does not prove direct channel binding.\nEvidence access: Primary full text\nGlycine-induced activation of GPR158 increases the intrinsic excitability of medium spiny neurons in the nucleus accumbens. · 2024 · https://pubmed.ncbi.nlm.nih.gov/38884814/ · DOI 10.1007/s00018-024-05260-w","model_system":"Male C57BL/6J mice aged four to six weeks; slice patch clamp with synaptic blockers.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"7b2859d8-e2bf-51e7-8e32-0715c10cbc49","stable_key":"import-507ed066-fd6d-5722-9223-97b66302e7e3","title":"Glycine: supply, one-carbon allocation, receptors and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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