{"id":"eccc4356-608f-55a6-b28e-4c2b6edaa987","stable_key":"ee4227e5-80d7-55e9-ada7-3c5a16f93ac9:cla-dgkh-knockdown","predicate":"knockdown_attenuates","statement":"DGK-eta siRNA reduced CLA-induced IL-8 and MCP-1 expression and JNK/c-Jun activation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"55b492ac-5fa1-58ed-ab2b-50d8d238b5aa","mechanism_event_label":"Gene depletion supported a contribution by a specific enzyme.","subject":{"id":"9d077e57-af75-5199-b040-b5c2acb02c7f","slug":"dgkh","display_name":"Human diacylglycerol kinase eta / DGKH","entity_type_key":"protein"},"object":{"id":"364992bb-f7d7-5e4d-967b-b6646faafd2b","slug":"human-adipocyte-cla-inflammatory-output","display_name":"Human adipocyte CLA-associated cytokine output","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"55b492ac-5fa1-58ed-ab2b-50d8d238b5aa","stable_key":"ee4227e5-80d7-55e9-ada7-3c5a16f93ac9:cla-dgkh-knockdown-event","event_type":"observed_relationship","label":"Gene depletion supported a contribution by a specific enzyme.","description":"DGK-eta siRNA reduced CLA-induced IL-8 and MCP-1 expression and JNK/c-Jun activation.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"9d077e57-af75-5199-b040-b5c2acb02c7f","slug":"dgkh","display_name":"Human diacylglycerol kinase eta / DGKH","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"364992bb-f7d7-5e4d-967b-b6646faafd2b","slug":"human-adipocyte-cla-inflammatory-output","display_name":"Human adipocyte CLA-associated cytokine output","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"5dd031e8-9ede-5db7-a4e1-961de31f0b49","slug":"cxcl8","display_name":"Human interleukin-8 / CXCL8","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"30896093-9180-5f67-800b-028fea02dcc8","slug":"ccl2","display_name":"Human monocyte chemoattractant protein-1 / CCL2","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"4405f918-c17d-5365-b873-1678989f8ac1","slug":"cla-trans10-cis12","display_name":"trans-10,cis-12 conjugated linoleic acid","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human adipocyte RNA-interference experiment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Partial effects do not establish an exclusive pathway.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"CLA collection; isomer, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"conjugated-linoleic-acid","display_name":"Conjugated linoleic acid / CLA isomer family","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"Gene depletion supported a contribution by a specific enzyme.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Diacylglycerol kinase inhibitor R59022 attenuates conjugated linoleic acid-mediated inflammation in human adipocytes. · 2013 · https://pubmed.ncbi.nlm.nih.gov/23264678/ · DOI 10.1194/jlr.M031211","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"b6182979-35ee-5f0b-94fd-275acd480505","evidence_kind":"source_excerpt","locator":"Lines 190-196","start_line":190,"end_line":196,"excerpt":"## cla-dgkh-knockdown\nGene depletion supported a contribution by a specific enzyme.\nDGK-eta siRNA reduced CLA-induced IL-8 and MCP-1 expression and JNK/c-Jun activation.\nModel: Human adipocyte RNA-interference experiment.\nLimitations: Partial effects do not establish an exclusive pathway.\nEvidence access: Primary abstract\nDiacylglycerol kinase inhibitor R59022 attenuates conjugated linoleic acid-mediated inflammation in human adipocytes. · 2013 · https://pubmed.ncbi.nlm.nih.gov/23264678/ · DOI 10.1194/jlr.M031211","model_system":"Human adipocyte RNA-interference experiment.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"45cfa6e3-2e7d-57c1-8c35-65302b7b71db","stable_key":"import-ee4227e5-80d7-55e9-ada7-3c5a16f93ac9","title":"Conjugated linoleic acid: isomers, signaling, nutrient interactions and discovery (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"f9fe73a8a4008d52061c65abe6de7a9806da75f1c478c5adace9387b8ffaad9e","revision_id":"21196d9c-aaab-5fe1-8f32-2987c94289c6","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}