{"id":"ecbfe98d-4626-5a56-9dce-7d59742bbda1","stable_key":"8600a864-6653-52c7-9718-1362c5af4577:gamma-nonalactone-motility-alone","predicate":"reduces","statement":"Gamma-nonalactone alone reduced planarian locomotion at higher concentrations, with an IC50 of 181 +/- 18 micromolar.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"3ebe1fbc-fef7-555e-a4ae-31e09896fb15","mechanism_event_label":"Gamma-nonalactone alone reduced planarian locomotion at higher concentrations, with an IC50 of 181 +/- 18 micromolar.","subject":{"id":"2b85fa4a-5c91-5de9-aead-a443bc4394af","slug":"gamma-nonalactone","display_name":"Gamma-nonalactone","entity_type_key":"small_molecule"},"object":{"id":"d490dd91-29b8-5008-b933-d21336d11df7","slug":"dugesia-tigrina-locomotion","display_name":"Locomotion of Dugesia tigrina planarians","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"3ebe1fbc-fef7-555e-a4ae-31e09896fb15","stable_key":"8600a864-6653-52c7-9718-1362c5af4577:gamma-nonalactone-motility-alone-event","event_type":"observed_relationship","label":"Gamma-nonalactone alone reduced planarian locomotion at higher concentrations, with an IC50 of 181 +/- 18 micromolar.","description":"Gamma-nonalactone alone reduced planarian locomotion at higher concentrations, with an IC50 of 181 +/- 18 micromolar.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"2b85fa4a-5c91-5de9-aead-a443bc4394af","slug":"gamma-nonalactone","display_name":"Gamma-nonalactone","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"d490dd91-29b8-5008-b933-d21336d11df7","slug":"dugesia-tigrina-locomotion","display_name":"Locomotion of Dugesia tigrina planarians","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"Gamma-nonalactone concentration series without cocaine","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"10 min incubation followed by 5 min recording","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary full-text methods/results inspected; PubMed metadata where indexed.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; model-specific source-derived curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Dugesia tigrina planarians","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Behavioral impairment is not direct evidence for DAT, GABA receptors, or a human toxic dose. Compound alone at 51 micromolar did not significantly change locomotion.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Gamma-nonalactone flavor-compound chapter; nutrient and drug interactions retain their experimental settings.","comparator":null,"unit":null,"notes":"","entity":{"slug":"gamma-nonalactone","display_name":"Gamma-nonalactone","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Dugesia tigrina planarians","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Gamma-nonalactone alone reduced planarian locomotion at higher concentrations, with an IC50 of 181 +/- 18 micromolar.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Minimal structural requirements of alkyl γ-lactones capable of antagonizing the cocaine-induced motility decrease in planarians. (2011). https://pubmed.ncbi.nlm.nih.gov/21878350/ DOI: 10.1016/j.pbb.2011.08.013","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"Whole-worm bath exposure","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Grid-crossing locomotion assay","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"9e142410-6675-54cb-9a33-45560cb76751","evidence_kind":"source_excerpt","locator":"Lines 224-233","start_line":224,"end_line":233,"excerpt":"## gamma-nonalactone-motility-alone\nGamma-nonalactone alone reduced planarian locomotion at higher concentrations, with an IC50 of 181 +/- 18 micromolar.\nModel/species: Dugesia tigrina planarians\nTissue: Grid-crossing locomotion assay\nExposure: Gamma-nonalactone concentration series without cocaine\nRoute: Whole-worm bath exposure\nDuration: 10 min incubation followed by 5 min recording\nLimits: Behavioral impairment is not direct evidence for DAT, GABA receptors, or a human toxic dose. Compound alone at 51 micromolar did not significantly change locomotion.\nPrimary reference: Minimal structural requirements of alkyl γ-lactones capable of antagonizing the cocaine-induced motility decrease in planarians. (2011). https://pubmed.ncbi.nlm.nih.gov/21878350/ DOI: 10.1016/j.pbb.2011.08.013\nAccess: Primary full-text methods/results inspected; PubMed metadata where indexed.","model_system":"Dugesia tigrina planarians","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span with primary citation; not a publisher quotation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"73777c54-95a4-5b07-9d14-d4dd31949fb1","stable_key":"import-8600a864-6653-52c7-9718-1362c5af4577","title":"Gamma-nonalactone: mechanisms, molecular forms and cross-actor connections (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted curation of eight primary studies. Study-specific citations, negative findings and limitations retained. Not publisher full text.","file_path":"","sha256":"a6fede6fbb2b70669be6846626f9c197edfd605c627d50fffa984e2b0b7ed71d","revision_id":"bb5ac9d2-d97d-59ac-a99c-ddf46d535574","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}