{"id":"ec993c7f-119a-5cb6-9c8b-dbf9c51b6700","stable_key":"dc8975b1-95ff-5d9b-be17-a1c04610cca7:mo-gphn-insertion","predicate":"incorporates","statement":"The gephyrin E domain catalyzes molybdate incorporation during ATP-dependent Moco synthesis.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"c4f4b498-0887-5475-8573-b98179a816eb","mechanism_event_label":"Molybdenum becomes useful to these enzymes only after being inserted into its cofactor.","subject":{"id":"417f91f9-e416-5947-abd7-7891452cac9e","slug":"gphn-e-domain","display_name":"Mammalian gephyrin E domain","entity_type_key":"protein"},"object":{"id":"18479412-e52f-5921-8b5a-ddacefcbe083","slug":"molybdate","display_name":"Molybdate / MoO4(2-)","entity_type_key":"ion"},"evidence_count":1,"mechanism_event":{"id":"c4f4b498-0887-5475-8573-b98179a816eb","stable_key":"dc8975b1-95ff-5d9b-be17-a1c04610cca7:mo-gphn-insertion-event","event_type":"biochemical_relationship","label":"Molybdenum becomes useful to these enzymes only after being inserted into its cofactor.","description":"The gephyrin E domain catalyzes molybdate incorporation during ATP-dependent Moco synthesis.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"545cec69-0265-5ab0-b1cc-58d5e7026196","slug":"mpt-amp","display_name":"Adenylylated molybdopterin / MPT-AMP","entity_type_key":"small_molecule"},"role":"activated scaffold","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"ac4bcc03-66fb-52c1-b191-9ecb2124659f","slug":"molybdenum-cofactor","display_name":"Molybdenum cofactor / Moco","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"f245ac90-f29d-57cd-a783-52793b7f07aa","slug":"gphn","display_name":"Human gephyrin / GPHN","entity_type_key":"protein"},"role":"full protein","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"36447b98-3392-559f-b76c-035726a2538e","slug":"molybdenum","display_name":"Molybdenum","entity_type_key":"nutrient_element"},"role":"element","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"417f91f9-e416-5947-abd7-7891452cac9e","slug":"gphn-e-domain","display_name":"Mammalian gephyrin E domain","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"18479412-e52f-5921-8b5a-ddacefcbe083","slug":"molybdate","display_name":"Molybdate / MoO4(2-)","entity_type_key":"ion"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/molybdenum-research/23163752.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"909388cf5fb7bfa5d2f8444644e20773a7a8ec6a19b4ccb8a4bda78d9ad17c5d\", \"start_char\": 0, \"end_char\": 1489, \"text_sha256\": \"909388cf5fb7bfa5d2f8444644e20773a7a8ec6a19b4ccb8a4bda78d9ad17c5d\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified mammalian gephyrin domains and full multidomain protein; in-vitro Moco reconstitution","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"MPT adenylation, molybdate insertion and domain-combination assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"No human dietary magnesium intervention; purified domain kinetics should not be turned into supplementation efficacy.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Molybdenum research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"molybdenum","display_name":"Molybdenum","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Mammalian protein system","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Molybdenum becomes useful to these enzymes only after being inserted into its cofactor.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[mo-p23163752] Metal insertion into the molybdenum cofactor: product-substrate channelling demonstrates the functional origin of domain fusion in gephyrin. (2013). https://pubmed.ncbi.nlm.nih.gov/23163752/ DOI: 10.1042/bj20121078","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified gephyrin","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"5902c1f6-c995-5831-8ad6-4f0e3fa9b795","evidence_kind":"source_excerpt","locator":"Lines 495-506","start_line":495,"end_line":506,"excerpt":"### mo-gphn-insertion\nThe gephyrin E domain catalyzes molybdate incorporation during ATP-dependent Moco synthesis.\nCondition category: normal\nnutrient_topic: Molybdenum research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Molybdenum becomes useful to these enzymes only after being inserted into its cofactor.\norganism: Mammalian protein system\ntissue_or_cell_type: Purified gephyrin\nexperimental_model: Purified mammalian gephyrin domains and full multidomain protein; in-vitro Moco reconstitution\nlimitations: No human dietary magnesium intervention; purified domain kinetics should not be turned into supplementation efficacy.\nexposure: MPT adenylation, molybdate insertion and domain-combination assays\nevidence_span: {\"source_cache\": \"artifacts/molybdenum-research/23163752.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"909388cf5fb7bfa5d2f8444644e20773a7a8ec6a19b4ccb8a4bda78d9ad17c5d\", \"start_char\": 0, \"end_char\": 1489, \"text_sha256\": \"909388cf5fb7bfa5d2f8444644e20773a7a8ec6a19b4ccb8a4bda78d9ad17c5d\"}\n[mo-p23163752] Metal insertion into the molybdenum cofactor: product-substrate channelling demonstrates the functional origin of domain fusion in gephyrin. (2013). https://pubmed.ncbi.nlm.nih.gov/23163752/ DOI: 10.1042/bj20121078","model_system":"Purified mammalian gephyrin domains and full multidomain protein; in-vitro Moco reconstitution","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [mo-p23163752] Metal insertion into the molybdenum cofactor: product-substrate channelling demonstrates the functional origin of domain fusion in gephyrin. (2013). https://pubmed.ncbi.nlm.nih.gov/23163752/ DOI: 10.1042/bj20121078","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"1aa6da60-8284-5252-8dd9-ac2250d5ced5","stable_key":"import-dc8975b1-95ff-5d9b-be17-a1c04610cca7","title":"Molybdenum: cofactor assembly, sulfur metabolism and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"143147eb9ac7fe750bdd33bb50d7773bc0cd49cc94faf219aebce3c3d760dd8f","revision_id":"90c2239d-290b-5e16-815a-f94d30569ebd","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}