{"id":"ec58df65-c3c7-5e18-b988-8fced522ce9e","stable_key":"1bbf9d44-8d2b-5b35-8f82-f6876558b0b3:cyp3a5-does-not-produce-am1","predicate":"does_not_produce","statement":"CYP3A5 did not form AM1 from cyclosporine in the same comparison in which CYP3A4 did.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"dbfa158a-4625-540e-973e-eef4d94481d3","mechanism_event_label":"CYP3A5 did not form AM1 from cyclosporine in the same comparison in which CYP3A4 did.","subject":{"id":"222f6587-14d7-5110-902a-f390498c3b12","slug":"cyp3a5","display_name":"Human cytochrome P450 3A5","entity_type_key":"protein"},"object":{"id":"382f21b3-d339-55f0-a280-c8a0902874be","slug":"cyclosporine-am1","display_name":"Cyclosporine metabolite AM1","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"dbfa158a-4625-540e-973e-eef4d94481d3","stable_key":"1bbf9d44-8d2b-5b35-8f82-f6876558b0b3:cyp3a5-does-not-produce-am1-event","event_type":"biochemical_relationship","label":"CYP3A5 did not form AM1 from cyclosporine in the same comparison in which CYP3A4 did.","description":"Oxidative drug metabolism","status":"provisional","compartment":null,"participants":[{"entity":{"id":"222f6587-14d7-5110-902a-f390498c3b12","slug":"cyp3a5","display_name":"Human cytochrome P450 3A5","entity_type_key":"protein"},"role":"acting component","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"382f21b3-d339-55f0-a280-c8a0902874be","slug":"cyclosporine-am1","display_name":"Cyclosporine metabolite AM1","entity_type_key":"small_molecule"},"role":"component the finding is about","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"duration","value_text":"Not stated here","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Heterologously expressed human CYP3A4 and CYP3A5","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Cyclosporin A","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"A measured absence in one recombinant system, not a statement that the reaction is impossible in tissue.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Heterologously expressed human CYP3A4 and CYP3A5","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"CYP3A5 did not form AM1 from cyclosporine in the same comparison in which CYP3A4 did.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"In vitro metabolism of cyclosporine A by human kidney CYP3A5. (2004). https://pubmed.ncbi.nlm.nih.gov/15450954/ DOI: 10.1016/j.bcp.2004.07.012","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Oxidative drug metabolism","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"261053c5-f630-52ae-8cc0-61a5df223ec6","evidence_kind":"source_excerpt","locator":"Lines 201-201","start_line":201,"end_line":201,"excerpt":"CYP3A5 did not form AM1 from cyclosporine in the same comparison in which CYP3A4 did.","model_system":"Heterologously expressed human CYP3A4 and CYP3A5","directness":"reported_statement","verification_status":"source_derived_draft","notes":"","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"6b2b7746-fc66-5491-b896-e6261df254c9","stable_key":"import-1bbf9d44-8d2b-5b35-8f82-f6876558b0b3","title":"Cyclosporine: the complex that inhibits calcineurin, a second cyclophilin, and the transport step that decides exposure (2026-09-23)","document_type":"imported_text","citation_label":"Original AI-assisted curation of seven primary studies resolved by PubMed title search, with every abstract read and all DOIs cross-checked against live PubMed metadata on 2026-09-23. No reference carries a recorded retraction, erratum or expression of concern. Each of the seven is a separate laboratory and each carries its own lineage key, so none of them can be counted twice as independent support. Study-specific concentrations, kinetic constants and limitations retained. Not publisher full text.","file_path":"","sha256":"499d00555a629cba2271c3888d7e93627949891d565c4c78c843610de2913080","revision_id":"263ee68a-4184-51d0-a977-197d7ddb7a4d","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}