{"id":"ec4cd52f-e205-5a4e-be7e-bb18711e4438","stable_key":"2921131d-0ba0-51d3-8f59-8c4be3b9ac08:cucurbitacin-i-canine-motility","predicate":"inhibits_tested","statement":"Adding cucurbitacin I rapidly reduced motility and produced actin aggregates in canine MDCK cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"d0d954ef-0ed6-5505-8a79-af072fd5e0f6","mechanism_event_label":"Cell movement changes along with filament organization.","subject":{"id":"55ef0ba6-495d-5cc4-88c5-0772ab25d0fe","slug":"cucurbitacin-i","display_name":"Cucurbitacin I","entity_type_key":"small_molecule"},"object":{"id":"11b2c425-0099-57ae-9b5f-cc7566e9023f","slug":"canine-mdck-cucurbitacin-motility","display_name":"Canine MDCK cell motility during cucurbitacin I exposure","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"d0d954ef-0ed6-5505-8a79-af072fd5e0f6","stable_key":"2921131d-0ba0-51d3-8f59-8c4be3b9ac08:cucurbitacin-i-canine-motility-event","event_type":"observed_relationship","label":"Cell movement changes along with filament organization.","description":"Adding cucurbitacin I rapidly reduced motility and produced actin aggregates in canine MDCK cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"55ef0ba6-495d-5cc4-88c5-0772ab25d0fe","slug":"cucurbitacin-i","display_name":"Cucurbitacin I","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"11b2c425-0099-57ae-9b5f-cc7566e9023f","slug":"canine-mdck-cucurbitacin-motility","display_name":"Canine MDCK cell motility during cucurbitacin I exposure","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text PMC2991314","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Canine MDCK cellular experiments; also mouse B16-F1 experiments in the paper.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cellular phenotype does not identify a direct molecular binding target.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Cucurbitacins collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"cucurbitacin","display_name":"Cucurbitacins","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"Cell movement changes along with filament organization.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Cucurbitacin I inhibits cell motility by indirectly interfering with actin dynamics. · 2010 · https://pubmed.ncbi.nlm.nih.gov/21124831/ · DOI 10.1371/journal.pone.0014039","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"3af557f6-3de5-5797-8c52-8f520271e650","evidence_kind":"source_excerpt","locator":"Lines 68-74","start_line":68,"end_line":74,"excerpt":"## cucurbitacin-i-canine-motility\nCell movement changes along with filament organization.\nAdding cucurbitacin I rapidly reduced motility and produced actin aggregates in canine MDCK cells.\nModel: Canine MDCK cellular experiments; also mouse B16-F1 experiments in the paper.\nLimitations: Cellular phenotype does not identify a direct molecular binding target.\nEvidence access: Primary full text PMC2991314\nCucurbitacin I inhibits cell motility by indirectly interfering with actin dynamics. · 2010 · https://pubmed.ncbi.nlm.nih.gov/21124831/ · DOI 10.1371/journal.pone.0014039","model_system":"Canine MDCK cellular experiments; also mouse B16-F1 experiments in the paper.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"77324310-00ce-5d28-9fc7-595fddae86b9","stable_key":"import-2921131d-0ba0-51d3-8f59-8c4be3b9ac08","title":"Cucurbitacins: thiol chemistry, cytoskeleton, metabolic dependencies and signaling (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"5c23ec61b9cf3d2966fb081d8617e1e1f7c522e86419b8ba2dcae09590121123","revision_id":"74251a06-3ba5-506c-b428-700eca0fb432","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}