{"id":"ec330c58-42df-5281-92b0-76906aedabbe","stable_key":"773f772e-bf8e-5e52-a2a2-6dd694d67f5d:bromelain-neutrophil-cxcr","predicate":"proteolytically_removes","statement":"Bromelain removed CXCR1/CXCR2 from human neutrophils and reduced IL-8-directed migration by about 40%.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"0ac62eb7-4edd-5982-9ef7-45eb33ed471a","mechanism_event_label":"Bromelain removed CXCR1/CXCR2 from human neutrophils and reduced IL-8-directed migration by about 40%.","subject":{"id":"6a3990e6-85af-58c7-8087-8a251db842fa","slug":"bromelain","display_name":"Bromelain","entity_type_key":"peptide"},"object":{"id":"29e94513-2962-5ed5-831e-8fc85608cdc4","slug":"human-cxcr1-cxcr2","display_name":"Human CXCR1/CXCR2 chemokine receptors","entity_type_key":"protein_set"},"evidence_count":1,"mechanism_event":{"id":"0ac62eb7-4edd-5982-9ef7-45eb33ed471a","stable_key":"773f772e-bf8e-5e52-a2a2-6dd694d67f5d:bromelain-neutrophil-cxcr-event","event_type":"observed_relationship","label":"Bromelain removed CXCR1/CXCR2 from human neutrophils and reduced IL-8-directed migration by about 40%.","description":"Bromelain removed CXCR1/CXCR2 from human neutrophils and reduced IL-8-directed migration by about 40%.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6a3990e6-85af-58c7-8087-8a251db842fa","slug":"bromelain","display_name":"Bromelain","entity_type_key":"peptide"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"29e94513-2962-5ed5-831e-8fc85608cdc4","slug":"human-cxcr1-cxcr2","display_name":"Human CXCR1/CXCR2 chemokine receptors","entity_type_key":"protein_set"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"5dd031e8-9ede-5db7-a4e1-961de31f0b49","slug":"cxcl8","display_name":"Human interleukin-8 / CXCL8","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"In-vitro bromelain treatment; in-vivo preparation specified in the primary study","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Acute","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; model-specific source-derived curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Isolated human neutrophils; three murine inflammation models","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The effect was stimulus-selective and does not imply global suppression of neutrophil migration or human clinical efficacy.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Bromelain chapter; interacting nutrients, drugs, peptides and proteins retain their experimental settings.","comparator":null,"unit":null,"notes":"","entity":{"slug":"bromelain","display_name":"Bromelain","entity_type_key":"peptide"}},{"dimension":"organism","value_text":"Isolated human neutrophils; three murine inflammation models","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Bromelain removed CXCR1/CXCR2 from human neutrophils and reduced IL-8-directed migration by about 40%.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Bromelain treatment decreases neutrophil migration to sites of inflammation. (2008). https://pubmed.ncbi.nlm.nih.gov/18482869/ DOI: 10.1016/j.clim.2008.02.015","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro and in vivo","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Chemokine-receptor surface expression and chemotaxis","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"eba85202-eb01-5e9a-82c9-26f695ecafc8","evidence_kind":"source_excerpt","locator":"Lines 33-42","start_line":33,"end_line":42,"excerpt":"## bromelain-neutrophil-cxcr\nBromelain removed CXCR1/CXCR2 from human neutrophils and reduced IL-8-directed migration by about 40%.\nModel/species: Isolated human neutrophils; three murine inflammation models\nTissue/system: Chemokine-receptor surface expression and chemotaxis\nExposure: In-vitro bromelain treatment; in-vivo preparation specified in the primary study\nRoute: In vitro and in vivo\nDuration: Acute\nLimits: The effect was stimulus-selective and does not imply global suppression of neutrophil migration or human clinical efficacy.\nPrimary reference: Bromelain treatment decreases neutrophil migration to sites of inflammation. (2008). https://pubmed.ncbi.nlm.nih.gov/18482869/ DOI: 10.1016/j.clim.2008.02.015\nAccess: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","model_system":"Isolated human neutrophils; three murine inflammation models","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span with primary citation; not a publisher quotation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"9d363b4e-f438-5664-9d74-99c2e359391d","stable_key":"import-773f772e-bf8e-5e52-a2a2-6dd694d67f5d","title":"Bromelain: mechanism of action and interactions (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted source-specific curation with primary-study citations, model, exposure, route, duration, negative findings and limitations preserved. Not publisher full text.","file_path":"","sha256":"b328dbb8082bea612bb2c40d6ff41fa670f6e62f714bd014c90627fcab86e95b","revision_id":"1c10d22e-ecdf-56ef-b820-bb21182a9577","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}