{"id":"ec271967-2622-5727-a74d-2715e57260b9","stable_key":"1ff3a29f-ef80-5bff-9300-30b98c60b90d:strontium-mouse-sealing-zone","predicate":"disrupts","statement":"Strontium ranelate disrupted the actin-containing sealing zone of mouse osteoclasts and reduced resorbing activity.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"d1487e91-9f46-5bc6-9014-c7d1efcb23a3","mechanism_event_label":"Changing the attachment structure can impair bone resorption.","subject":{"id":"a319eca8-b0ed-5a8c-8659-e7f106bed73a","slug":"strontium-ranelate","display_name":"Strontium ranelate","entity_type_key":"chemical_species"},"object":{"id":"ab042f8f-6cdb-522b-99be-4c6fbea23ac3","slug":"mouse-osteoclast-actin-sealing-zone","display_name":"Actin-containing sealing zone in mouse osteoclasts","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"d1487e91-9f46-5bc6-9014-c7d1efcb23a3","stable_key":"1ff3a29f-ef80-5bff-9300-30b98c60b90d:strontium-mouse-sealing-zone-event","event_type":"observed_relationship","label":"Changing the attachment structure can impair bone resorption.","description":"Strontium ranelate disrupted the actin-containing sealing zone of mouse osteoclasts and reduced resorbing activity.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"a319eca8-b0ed-5a8c-8659-e7f106bed73a","slug":"strontium-ranelate","display_name":"Strontium ranelate","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"ab042f8f-6cdb-522b-99be-4c6fbea23ac3","slug":"mouse-osteoclast-actin-sealing-zone","display_name":"Actin-containing sealing zone in mouse osteoclasts","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"2ca2864b-1ef0-54e4-8397-1dbe7207a0d2","slug":"strontium","display_name":"Strontium","entity_type_key":"nutrient_element"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Primary murine spleen-derived osteoclast culture.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Distinct from merely reducing osteoclast numbers; experimental concentration limits apply.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Strontium collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"strontium","display_name":"Strontium","entity_type_key":"nutrient_element"}},{"dimension":"plain_language","value_text":"Changing the attachment structure can impair bone resorption.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Dual effect of strontium ranelate: stimulation of osteoblast differentiation and inhibition of osteoclast formation and resorption in vitro. · 2008 · https://pubmed.ncbi.nlm.nih.gov/17945546/ · DOI 10.1016/j.bone.2007.08.043","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"e0a57d7e-32a3-564e-8b10-e87fc3cbc0ef","evidence_kind":"source_excerpt","locator":"Lines 246-252","start_line":246,"end_line":252,"excerpt":"## strontium-mouse-sealing-zone\nChanging the attachment structure can impair bone resorption.\nStrontium ranelate disrupted the actin-containing sealing zone of mouse osteoclasts and reduced resorbing activity.\nModel: Primary murine spleen-derived osteoclast culture.\nLimitations: Distinct from merely reducing osteoclast numbers; experimental concentration limits apply.\nEvidence access: Primary abstract\nDual effect of strontium ranelate: stimulation of osteoblast differentiation and inhibition of osteoclast formation and resorption in vitro. · 2008 · https://pubmed.ncbi.nlm.nih.gov/17945546/ · DOI 10.1016/j.bone.2007.08.043","model_system":"Primary murine spleen-derived osteoclast culture.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"43ef0fe7-eb6c-5346-8381-f7694733b4dc","stable_key":"import-1ff3a29f-ef80-5bff-9300-30b98c60b90d","title":"Strontium: calcium interactions, cellular mechanisms and mineralization (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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