{"id":"ec1354c2-4fa4-5212-85d0-9b906b54c400","stable_key":"52871722-4aa6-5453-a902-3e76356db327:resveratrol-mouse-camp","predicate":"increases","statement":"Resveratrol increased cAMP in mouse C2C12 myotubes at concentrations up to 50 micromolar; oral gavage also increased measured muscle and adipose cAMP in mice.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"182f3e47-51d7-5b98-a7d5-6cbb24d50033","mechanism_event_label":"Reduced signal breakdown connects enzyme inhibition to cellular signaling.","subject":{"id":"53549e32-e6b4-5f09-94e6-62867b015713","slug":"resveratrol","display_name":"Resveratrol","entity_type_key":"small_molecule"},"object":{"id":"d0776a22-262f-5258-ad32-472bbe1e6dad","slug":"mouse-c2c12-camp-content","display_name":"Cyclic AMP content in mouse C2C12 cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"182f3e47-51d7-5b98-a7d5-6cbb24d50033","stable_key":"52871722-4aa6-5453-a902-3e76356db327:resveratrol-mouse-camp-event","event_type":"observed_relationship","label":"Reduced signal breakdown connects enzyme inhibition to cellular signaling.","description":"Resveratrol increased cAMP in mouse C2C12 myotubes at concentrations up to 50 micromolar; oral gavage also increased measured muscle and adipose cAMP in mice.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"53549e32-e6b4-5f09-94e6-62867b015713","slug":"resveratrol","display_name":"Resveratrol","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"d0776a22-262f-5258-ad32-472bbe1e6dad","slug":"mouse-c2c12-camp-content","display_name":"Cyclic AMP content in mouse C2C12 cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"9cc3323e-3a7f-5be5-91ae-78dab284fb81","slug":"cyclic-amp","display_name":"Cyclic adenosine monophosphate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse cells and separate mouse tissue measurements.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"A changed pool does not by itself identify its synthesis or breakdown rate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Resveratrol collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"resveratrol","display_name":"Resveratrol","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Reduced signal breakdown connects enzyme inhibition to cellular signaling.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Resveratrol ameliorates aging-related metabolic phenotypes by inhibiting cAMP phosphodiesterases. · 2012 · https://pubmed.ncbi.nlm.nih.gov/22304913/ · DOI 10.1016/j.cell.2012.01.017","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"d2267fe1-5ea3-550e-abc3-624c18eae74c","evidence_kind":"source_excerpt","locator":"Lines 198-204","start_line":198,"end_line":204,"excerpt":"## resveratrol-mouse-camp\nReduced signal breakdown connects enzyme inhibition to cellular signaling.\nResveratrol increased cAMP in mouse C2C12 myotubes at concentrations up to 50 micromolar; oral gavage also increased measured muscle and adipose cAMP in mice.\nModel: Mouse cells and separate mouse tissue measurements.\nLimitations: A changed pool does not by itself identify its synthesis or breakdown rate.\nEvidence access: Primary full text\nResveratrol ameliorates aging-related metabolic phenotypes by inhibiting cAMP phosphodiesterases. · 2012 · https://pubmed.ncbi.nlm.nih.gov/22304913/ · DOI 10.1016/j.cell.2012.01.017","model_system":"Mouse cells and separate mouse tissue measurements.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2844eda9-a122-5357-ae47-cd58227c5994","stable_key":"import-52871722-4aa6-5453-a902-3e76356db327","title":"Resveratrol: metabolites, target selectivity and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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