{"id":"ec12e0d3-ac11-529a-b3f6-ccaaa7e5b4f0","stable_key":"79018983-9179-5c1a-8e7a-d6f47a13582b:ki-lpo-activate","predicate":"forms","statement":"H2O2 formed LPO compound I with second-order rate constant (1.1 ± 0.1) × 10^7 M−1 s−1.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"d0fa8be1-a624-5bdd-8855-d0323c52aff1","mechanism_event_label":"Peroxide activates the enzyme before iodide oxidation.","subject":{"id":"da9d64bc-69d4-5d97-90a4-8f0ed03e0ac8","slug":"hydrogen-peroxide","display_name":"Hydrogen peroxide","entity_type_key":"small_molecule"},"object":{"id":"0745f5ac-0f33-5adc-be03-c626ccb8f3ff","slug":"lpo-compound-i","display_name":"Lactoperoxidase compound I","entity_type_key":"protein_state"},"evidence_count":1,"mechanism_event":{"id":"d0fa8be1-a624-5bdd-8855-d0323c52aff1","stable_key":"79018983-9179-5c1a-8e7a-d6f47a13582b:ki-lpo-activate-event","event_type":"observed_relationship","label":"Peroxide activates the enzyme before iodide oxidation.","description":"H2O2 formed LPO compound I with second-order rate constant (1.1 ± 0.1) × 10^7 M−1 s−1.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"da9d64bc-69d4-5d97-90a4-8f0ed03e0ac8","slug":"hydrogen-peroxide","display_name":"Hydrogen peroxide","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"0745f5ac-0f33-5adc-be03-c626ccb8f3ff","slug":"lpo-compound-i","display_name":"Lactoperoxidase compound I","entity_type_key":"protein_state"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"3d671567-4fb6-5b5f-a328-156987d5b396","slug":"lactoperoxidase-family","display_name":"Lactoperoxidase enzyme family","entity_type_key":"protein_family"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Purified LPO transient kinetics; pH 7, 15 °C; preparation species unresolved in abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure_category","value_text":"Study-specific exposure, including pharmacological and in-vitro conditions; normal is the schema fallback outside the three availability categories.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Purified enzyme kinetics; concentrations, substrate competition and reaction order determine relevance in tissues.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Potassium iodide research collection; shared-anion and comparator studies are not all KI interventions.","comparator":null,"unit":null,"notes":"","entity":{"slug":"potassium-iodide","display_name":"Potassium iodide","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"Peroxide activates the enzyme before iodide oxidation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Reaction of lactoperoxidase compound I with halides and thiocyanate. · 2002 · https://pubmed.ncbi.nlm.nih.gov/12269834/ · DOI 10.1021/bi026326x","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"79fbbd08-81d7-5a89-aaaf-c9e00026ac8a","evidence_kind":"source_excerpt","locator":"Lines 184-190","start_line":184,"end_line":190,"excerpt":"## ki-lpo-activate\nPeroxide activates the enzyme before iodide oxidation.\nH2O2 formed LPO compound I with second-order rate constant (1.1 ± 0.1) × 10^7 M−1 s−1.\nModel: Purified LPO transient kinetics; pH 7, 15 °C; preparation species unresolved in abstract.\nLimitations: Purified enzyme kinetics; concentrations, substrate competition and reaction order determine relevance in tissues.\nEvidence location: Primary abstract\nReaction of lactoperoxidase compound I with halides and thiocyanate. · 2002 · https://pubmed.ncbi.nlm.nih.gov/12269834/ · DOI 10.1021/bi026326x","model_system":"Purified LPO transient kinetics; pH 7, 15 °C; preparation species unresolved in abstract.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Curation paraphrase; primary evidence location and source kind retained above.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dc3ce411-f68f-5eb8-87c1-1592665f9cb6","stable_key":"import-79018983-9179-5c1a-8e7a-d6f47a13582b","title":"Potassium iodide: thyroid and non-thyroid mechanisms, interactions and discovery questions (2026-09-18)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-study references, chemical references and label statements individually identified. 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