{"id":"ec0e35e5-058d-5595-a4f2-f9417c81a06a","stable_key":"59b18080-c560-563d-abc5-bac4ee82a27c:ergothioneine-endothelial-gsr","predicate":"increases_expression","statement":"Ergothioneine increased glutathione reductase expression in human brain microvascular endothelial cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"cfe95b86-d97d-57bf-a1bf-ed8857276499","mechanism_event_label":"This connects the response to glutathione recycling.","subject":{"id":"939c92c3-50ea-5a4a-9654-8e3b94448132","slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"},"object":{"id":"ae04c12f-c0ac-5c8d-9ee2-76e58156d105","slug":"gsr","display_name":"Glutathione reductase / GSR","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"cfe95b86-d97d-57bf-a1bf-ed8857276499","stable_key":"59b18080-c560-563d-abc5-bac4ee82a27c:ergothioneine-endothelial-gsr-event","event_type":"observed_relationship","label":"This connects the response to glutathione recycling.","description":"Ergothioneine increased glutathione reductase expression in human brain microvascular endothelial cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"939c92c3-50ea-5a4a-9654-8e3b94448132","slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"ae04c12f-c0ac-5c8d-9ee2-76e58156d105","slug":"gsr","display_name":"Glutathione reductase / GSR","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"1d9b9d7b-3fdf-57af-b9dd-00194fa1de9e","slug":"oxidized-glutathione","display_name":"GSSG","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Cultured human endothelial cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"GSR expression does not establish increased flux or correction of riboflavin deficiency.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Ergothioneine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"This connects the response to glutathione recycling.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Uptake and protective effects of ergothioneine in human endothelial cells. · 2014 · https://pubmed.ncbi.nlm.nih.gov/25022513/ · DOI 10.1124/jpet.114.214049","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"a997d7d0-95c7-5994-8e7f-31c5441f5768","evidence_kind":"source_excerpt","locator":"Lines 296-302","start_line":296,"end_line":302,"excerpt":"## ergothioneine-endothelial-gsr\nThis connects the response to glutathione recycling.\nErgothioneine increased glutathione reductase expression in human brain microvascular endothelial cells.\nModel: Cultured human endothelial cells.\nLimitations: GSR expression does not establish increased flux or correction of riboflavin deficiency.\nEvidence access: Primary abstract\nUptake and protective effects of ergothioneine in human endothelial cells. · 2014 · https://pubmed.ncbi.nlm.nih.gov/25022513/ · DOI 10.1124/jpet.114.214049","model_system":"Cultured human endothelial cells.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2f037d98-f3d0-5dbe-80d8-90b738f130d6","stable_key":"import-59b18080-c560-563d-abc5-bac4ee82a27c","title":"Ergothioneine: transport, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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