{"id":"ebb83b71-c91f-51b0-8124-b32f52f9ae92","stable_key":"d2e91bc7-8177-5ad3-ad9b-5d65813ce082:naringenin-sirt1-silencing","predicate":"diminishes","statement":"SIRT1 siRNA diminished naringenin-associated improvements in endothelial senescence, oxidative stress and mitochondrial injury.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"2b2377ec-5668-5b13-9017-ea304b0af978","mechanism_event_label":"SIRT1 siRNA diminished naringenin-associated improvements in endothelial senescence, oxidative stress and mitochondrial injury.","subject":{"id":"ca5ef413-8800-5b6c-9942-9e2fa653d414","slug":"sirt1-sirna","display_name":"Experimental SIRT1 siRNA suppression","entity_type_key":"cellular_process"},"object":{"id":"967e1495-2667-5da3-b179-d1a8c50af263","slug":"naringenin-endothelial-senescence-response","display_name":"Naringenin response in oxidatively stressed endothelial cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"2b2377ec-5668-5b13-9017-ea304b0af978","stable_key":"d2e91bc7-8177-5ad3-ad9b-5d65813ce082:naringenin-sirt1-silencing-event","event_type":"observed_relationship","label":"SIRT1 siRNA diminished naringenin-associated improvements in endothelial senescence, oxidative stress and mitochondrial injury.","description":"SIRT1 siRNA diminished naringenin-associated improvements in endothelial senescence, oxidative stress and mitochondrial injury.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ca5ef413-8800-5b6c-9942-9e2fa653d414","slug":"sirt1-sirna","display_name":"Experimental SIRT1 siRNA suppression","entity_type_key":"cellular_process"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"967e1495-2667-5da3-b179-d1a8c50af263","slug":"naringenin-endothelial-senescence-response","display_name":"Naringenin response in oxidatively stressed endothelial cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"2a9350f6-01cc-5bea-8213-cd3ebb1b05cf","slug":"naringenin","display_name":"Naringenin","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"b8e1bfa9-c412-5f35-97af-cfc722123d27","slug":"sirt1","display_name":"SIRT1","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"Naringenin with SIRT1 siRNA","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Cell experiment accompanying three-month mouse dosing","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; model-specific source-derived curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Hydrogen-peroxide-stressed endothelial cells; aged ApoE-null mouse arm","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"SIRT1 involvement in a cell model does not establish direct SIRT1 binding or human atherosclerosis treatment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Naringenin chapter; interacting nutrients, drugs, peptides and proteins retain their experimental settings.","comparator":null,"unit":null,"notes":"","entity":{"slug":"naringenin","display_name":"Naringenin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Hydrogen-peroxide-stressed endothelial cells; aged ApoE-null mouse arm","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"SIRT1 siRNA diminished naringenin-associated improvements in endothelial senescence, oxidative stress and mitochondrial injury.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Naringenin ameliorates vascular senescence and atherosclerosis involving SIRT1 activation. (2023). https://pubmed.ncbi.nlm.nih.gov/37343148/ DOI: 10.1093/jpp/rgad053","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro perturbation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"SIRT1, FOXO3a/PGC1alpha and senescence measures","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"e5bfa6fa-5bba-52f4-862b-2bb8126c6c11","evidence_kind":"source_excerpt","locator":"Lines 143-152","start_line":143,"end_line":152,"excerpt":"## naringenin-sirt1-silencing\nSIRT1 siRNA diminished naringenin-associated improvements in endothelial senescence, oxidative stress and mitochondrial injury.\nModel/species: Hydrogen-peroxide-stressed endothelial cells; aged ApoE-null mouse arm\nTissue/system: SIRT1, FOXO3a/PGC1alpha and senescence measures\nExposure: Naringenin with SIRT1 siRNA\nRoute: In vitro perturbation\nDuration: Cell experiment accompanying three-month mouse dosing\nLimits: SIRT1 involvement in a cell model does not establish direct SIRT1 binding or human atherosclerosis treatment.\nPrimary reference: Naringenin ameliorates vascular senescence and atherosclerosis involving SIRT1 activation. (2023). https://pubmed.ncbi.nlm.nih.gov/37343148/ DOI: 10.1093/jpp/rgad053\nAccess: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","model_system":"Hydrogen-peroxide-stressed endothelial cells; aged ApoE-null mouse arm","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span with primary citation; not a publisher quotation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2804932d-46eb-5c43-8a45-0505e6b1ee05","stable_key":"import-d2e91bc7-8177-5ad3-ad9b-5d65813ce082","title":"Naringenin: mechanism of action and interactions (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted source-specific curation with primary-study citations, model, exposure, route, duration, negative findings and limitations preserved. Not publisher full text.","file_path":"","sha256":"e74776bf312a9ab53a4510c483e36e3093829e8c38725b9ae061e59e86f1145a","revision_id":"b96b5487-2b33-56ff-8955-9e8c0833533b","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}