{"id":"eb9be428-40d8-55ee-aa9d-b7763e45fe72","stable_key":"c9aa15a1-8ba2-5913-b2dd-372af304bf87:fast-hmgcl-magnesium","predicate":"coordinates_catalytic_complex","statement":"In HMGCL ternary structures Mg2+ coordinates His233, His235, Asp42, water and substrate/inhibitor oxygen atoms.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"1608d97c-9e77-510a-9e1c-cd8d96320523","mechanism_event_label":"Magnesium participates directly in this ketone-producing enzyme.","subject":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"object":{"id":"d2661983-120d-5a42-8ba3-454ab4727ed7","slug":"hmgcl","display_name":"Human HMG-CoA lyase / HMGCL","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"1608d97c-9e77-510a-9e1c-cd8d96320523","stable_key":"c9aa15a1-8ba2-5913-b2dd-372af304bf87:fast-hmgcl-magnesium-event","event_type":"observed_relationship","label":"Magnesium participates directly in this ketone-producing enzyme.","description":"In HMGCL ternary structures Mg2+ coordinates His233, His235, Asp42, water and substrate/inhibitor oxygen atoms.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"d2661983-120d-5a42-8ba3-454ab4727ed7","slug":"hmgcl","display_name":"Human HMG-CoA lyase / HMGCL","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"81e5bb47-0c11-54c9-a0ec-5c213930c173","slug":"hmg-coa","display_name":"(S)-3-Hydroxy-3-methylglutaryl-CoA","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human wild-type inhibitor complex and R41M substrate complex.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Structural dependence does not show that dietary magnesium limits ketosis in a replete person.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Fasting physiological-state collection; human protocols, cellular deprivation and refeeding are distinguished.","comparator":null,"unit":null,"notes":"","entity":{"slug":"fasting","display_name":"Fasting / abstention from energy intake","entity_type_key":"cellular_process"}},{"dimension":"plain_language","value_text":"Magnesium participates directly in this ketone-producing enzyme.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Functional insights into human HMG-CoA lyase from structures of Acyl-CoA-containing ternary complexes. · 2010 · https://pubmed.ncbi.nlm.nih.gov/20558737/ · DOI 10.1074/jbc.M110.139931","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"dee2420c-d900-58a6-bd19-7c3469ef954b","evidence_kind":"source_excerpt","locator":"Lines 120-126","start_line":120,"end_line":126,"excerpt":"## fast-hmgcl-magnesium\nMagnesium participates directly in this ketone-producing enzyme.\nIn HMGCL ternary structures Mg2+ coordinates His233, His235, Asp42, water and substrate/inhibitor oxygen atoms.\nModel: Human wild-type inhibitor complex and R41M substrate complex.\nLimitations: Structural dependence does not show that dietary magnesium limits ketosis in a replete person.\nEvidence access: Primary abstract\nFunctional insights into human HMG-CoA lyase from structures of Acyl-CoA-containing ternary complexes. · 2010 · https://pubmed.ncbi.nlm.nih.gov/20558737/ · DOI 10.1074/jbc.M110.139931","model_system":"Human wild-type inhibitor complex and R41M substrate complex.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"921d1eb5-2c88-52b9-9898-60b2b5bf6029","stable_key":"import-c9aa15a1-8ba2-5913-b2dd-372af304bf87","title":"Fasting: fuel switching, nutrient sensing, ketone signaling, nutrient dependencies and refeeding (2026-09-18)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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